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- Objective To establish a transient expression system of mouse DVL-1cDNA recombinant plasmid in cultured wild-type mouse Neuroblastoma 2a (N2a), for the further use in studying the role of Wnt signaling in Alzheimer disease pathogenesis. 目的 将鼠dishevelled 1(DVL 1)cDNA重组质粒转染到培养的鼠成神经瘤细胞N2a ,建立瞬时表达系统 ,为研究Wnt信号途径在阿尔茨海默病 (AD)发病中的作用提供实验基础。
- Methods: The transient expression system of COS7 was constructed by using pcDNA3.1-sEGFR501 expression vector and the process of Calcium-phosphate co-precipitation transfection was optimized with Enhanced Green Fluorescent Protein (EGFP) as a reporter. 方法:以增强型绿色荧光蛋白(EGFP)基因为报告基因,优化磷酸钙共沉淀转染COS7的实验参数; 以pcDNA3.;1-sEGFR501和COS7组建瞬时表达系统。
- As SV40 plasmids replicate out of control in COS7 cells and be lethal to such transfected cells, SV40 vector/COS7 cell system has only been using as transient expression system for a long time. 基于猴病毒40(SV40)载体在COS7细胞内的复制没有节制并对细胞具有致死性,长期以来SV40载体-COS7系统只被用作瞬时表达。
- The results suggested that the PEBV transient expression system has been established successfully and the GFP gene has been spread systemically and expressed at high level in Bean Pod. 结果表明,本实验成功地建立并优化了以PEBV为载体的外源蛋白瞬时表达体系,报告基因gfp实现了在豆角中的系统扩散和大量表达。
- On The Application Of Transient Expression System Of Protoplasm In Research Of Gene Expression 原生质体瞬间表达系统在基因表达研究中的应用
- Analysis of the Functions of Wheat Resistance-related Genes by a Transient Expression System 利用瞬间表达技术分析小麦抗病相关基因的功能
- transient expression system 瞬时表达系统
- Aim To construct the Pichia pastoris expression system of RS gene. 目的建立白藜芦醇合酶基因的毕赤酵母表达系统。
- Keywords Thinopyrum intermedium;wheat;Ae.squarrosa;Blumeria graminis;R..cerealisis;NPR1 homolog genes TiNH1;regulative response;Transient expression system;retrotransposons;transcription; 中间偃麦草;粗山羊草;小麦;小麦白粉病菌;小麦纹枯病菌;NPR1类似基因NH1;调控反应;瞬间表达系统;增强抗性;反转录转座子;转录;
- Increasing the Transient Expression of GUS Gene in Plant Tissue by Homologous Recombination. 利用同源重组提高外源GUS基因在植物组织中表达。
- The COS cells were transinfected by pC-IL-2 using DEAE-Dextran method for transient expression of IL-2 gene. 采用DEAE-Dextran介导的方法,用IL-2真核表达载体pC-IL-2转染COS细胞进行暂态表达。
- But MARs had no effect on the GUS gene expression levels in transient expression. 但在瞬时表达中 ,MARs对GUS基因的表达没有影响。
- The results were that transient expression had not obtained after transformating dry seeds implanted. 结果表明:直接注入干种子后进行转化,未得到瞬间表达的结果。
- Transient expression of the RZF71-GFP protein in onion epidermal cell showed that RZF71 was localized in cell nuclei. 农杆菌介导的洋葱表皮细胞GFP瞬时表达实验表明:RZF71定位于细胞核内。
- Single-chain Fv has mature prokaryocyte expression system with high yield, but its stability is poor. 单链抗体有较成熟的原核表达系统,产量较高,但稳定性较差。
- To improve this, the thesis researched the non-lytic baculovirus expression system by two different ways.First, Dr. 为了改善这个缺点,本论文由两个方向研究非溶裂杆状病毒表现系统。
- Methlotrophic yeast and fission yeast S.pombe have received attention as a good foreign genes expression system. 甲醇营养型酵母和裂殖酵母作为外源基因表达的有效系统,正引起人们广泛研究。
- The transient expression vector--pUC-pBin was built, the gene VirD_1, VirD_2, VirE_2 and GFP was joined in it respectively. 构建了瞬时表达载体pUC-pBin,并将目的基因VirD_1,VirD_2,VirE_2和GFP分别连在瞬时表达载体上。
- To improve this, the thesis researched the non-lytic baculovirus expression system by two different ways. 为了改善这个缺点,本论文由两个方向研究非溶裂杆状病毒表现系统。
- Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation. 将启动子片段分别以不同方向与gus报告基因和nos终止子融合 ,构建了瞬时表达载体。