The transient expression vector--pUC-pBin was built, the gene VirD_1, VirD_2, VirE_2 and GFP was joined in it respectively.

• 构建了瞬时表达载体pUC-pBin，并将目的基因VirD_1，VirD_2，VirE_2和GFP分别连在瞬时表达载体上。