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- transient expression activity 瞬时表达活性
- Increasing the Transient Expression of GUS Gene in Plant Tissue by Homologous Recombination. 利用同源重组提高外源GUS基因在植物组织中表达。
- The COS cells were transinfected by pC-IL-2 using DEAE-Dextran method for transient expression of IL-2 gene. 采用DEAE-Dextran介导的方法,用IL-2真核表达载体pC-IL-2转染COS细胞进行暂态表达。
- But MARs had no effect on the GUS gene expression levels in transient expression. 但在瞬时表达中 ,MARs对GUS基因的表达没有影响。
- The results were that transient expression had not obtained after transformating dry seeds implanted. 结果表明:直接注入干种子后进行转化,未得到瞬间表达的结果。
- Expression Activity of Cotton Leaf Curl Virus Large Intergenic Region in Host Plants. 棉花曲叶病毒大基因间区启动子在宿主植物中的表达活性。
- Transient expression of the RZF71-GFP protein in onion epidermal cell showed that RZF71 was localized in cell nuclei. 农杆菌介导的洋葱表皮细胞GFP瞬时表达实验表明:RZF71定位于细胞核内。
- The socialism with Chinese characteristics has an establishment, development and consummation in the interest expression activity. 中国特色社会主义就是在利益表达活动中得以确立、发展和完善的。
- The transient expression vector--pUC-pBin was built, the gene VirD_1, VirD_2, VirE_2 and GFP was joined in it respectively. 构建了瞬时表达载体pUC-pBin,并将目的基因VirD_1,VirD_2,VirE_2和GFP分别连在瞬时表达载体上。
- Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation. 将启动子片段分别以不同方向与gus报告基因和nos终止子融合 ,构建了瞬时表达载体。
- By detecting the transient expression of GUS gene, the factors affecting the early phase of Agrobacterium-mediated transformation were investigated. 通过测定GUS基因的瞬时表达率,对影响根癌农杆菌介导的香蕉基因转化早期主要因素进行了研究。
- By fusing this two fragment with gus reporter gene and nopaline terminator, we get two transient expression vectors which named pCAMBIA-ACTP and pBI-ACTPS. 将该启动子两个片段分别与gus报告基因和nos终止子融合,构建成瞬时植物表达载体pCAMBIA-ACTP和pBI-ACTPS。
- But if 2-day-seedling was implanted, and then dipped into DNA solution, the transformation effect was increased evidently. 10% transient expression was obtainted. 而注入玻璃化保护后的2天龄幼苗,再进行转化,转化效果明显提高,得到了大约10%25的瞬间表达。
- Comparing the CatO2 expressing activity of the two plasmids, we have discovered that the T32 terminator can effectively enhance the expression of foreign genes in Corynebacteria. 比较两个质粒的邻苯二酚双加氧酶表达活性,发现T32可在棒状杆菌中有效地促进外源基因的表达.
- Of the expressive activity and is ordered according to linguistic characteristics. 是表达行为的部分。并且,根据语言学上的特征,这综合有规则的。
- As SV40 plasmids replicate out of control in COS7 cells and be lethal to such transfected cells, SV40 vector/COS7 cell system has only been using as transient expression system for a long time. 基于猴病毒40(SV40)载体在COS7细胞内的复制没有节制并对细胞具有致死性,长期以来SV40载体-COS7系统只被用作瞬时表达。
- Objective To establish a transient expression system of mouse DVL-1cDNA recombinant plasmid in cultured wild-type mouse Neuroblastoma 2a (N2a), for the further use in studying the role of Wnt signaling in Alzheimer disease pathogenesis. 目的 将鼠dishevelled 1(DVL 1)cDNA重组质粒转染到培养的鼠成神经瘤细胞N2a ,建立瞬时表达系统 ,为研究Wnt信号途径在阿尔茨海默病 (AD)发病中的作用提供实验基础。
- Exect gene setuence hVEGF165 was obtained from the fetal human myocardium tissue by RT PCR; pcDNA3.1/VEGF165 was constructed ; and transient expression was going after transfecting COS 7 cell. RT PCR方法从胎儿心肌组织获得正确的hVEGF165基因序列,成功构建pcDNA3·1 /hVEGF165且实现转染COS 7细胞的瞬时表达。
- Methods: The transient expression system of COS7 was constructed by using pcDNA3.1-sEGFR501 expression vector and the process of Calcium-phosphate co-precipitation transfection was optimized with Enhanced Green Fluorescent Protein (EGFP) as a reporter. 方法:以增强型绿色荧光蛋白(EGFP)基因为报告基因,优化磷酸钙共沉淀转染COS7的实验参数; 以pcDNA3.;1-sEGFR501和COS7组建瞬时表达系统。
- Two recombinant human granulocyte colonystimulating factor expression plasmids pED-GCSF and pEF-GCSF were constructed. Each plasmid was transfected into the COS7 cells for transient expression,and into CHO-dhfr - cells for stable expression. 利用PCR反应、DNA测序、基因重组等技术,构建了两个表达人粒细胞集落刺激因子cDNA的重组质粒pED-GCSF和pEF-GCSF,两质粒分别转染COS7细胞作瞬时表达,转染CHO-dhfr-细胞作稳定表达。