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- As a mammalian gene transfer vector, baculovirus owns some unique advantages. 杆状病毒作为哺乳动物细胞基因转移的载体有很多独特的优点。
- LacZ gene was inserted into TK gene deleted transfer vector, the TK /LacZ+ /GTPV-TY mutant was screened. 将LacZ基因表达盒插入TK基因缺失转移载体,筛选TK基因缺失的GTPV-TY突变株。
- The resulting transfer vector pdTK-CMB can be used to expess genes from other bovine viruses (e.g. BPIV3> BRSV, BVDV) . 再将含SV40启动子的LacZ报告基因也亚克隆到该载体获得ndTKthacZ--G转移载体。
- Gene transfer vector is the important ingredient in gene therapy,which includes virus vector and non-virus vector. 基因转移载体是基因治疗的重要组成部分,包括病毒载体和非病毒载体。
- Thirdly, the application of BEM (Boundary Element Method) and ATV(Acoustic Transfer Vector) to the noise analysis of passenger are introduced. 随后,介绍了边界元法和声传递向量在车内声学中的应用。
- VP1 and VP2 genes of chicken anaemia virus were cloned into transfer vector pBacPAK8, then recombinant transfer plasmids pBac vp1 and pBac vp2 were obtained, respectively. 将鸡贫血病毒 VP1和 VP2基因分别克隆入转移载体 p Bac PAK8中 ,获得重组转移质粒 p Bac-vp1和 p Bac-vp2。
- Based on the acoustic transfer vector concept(ATV),the application approach to structural acoustic radiated prediction using the ATV technology for I.C.engine was studied. 基于声传递向量(ATV)的概念,研究探讨了ATV技术在内燃机结构辐射噪声仿真预测上的应用方法和途径。
- The invention provides a composition comprising a gene transfer vector which comprises a nucleic acid sequence encoding a protein and a carrier therefore. 本发明提供一合成物,内含基因转移载体,此载体含一组核酸序列,故能对蛋白质和隐性载体编码。
- To construct the bivalent genetic engineering vaccine against Pseudorabies and Foot and Mouth disease, P1 gene of foot-and mouth disease virus was inserted into a PRV universal vector-PgG-uni, and transfer vector PgG-P1 was constructed. 为了构建口蹄疫与伪狂犬病二价基因工程疫苗株,将口蹄疫病毒(Foot-and-mouth disease virus,FMDV)P1基因插入到载体pPgG-uni中,得到伪狂犬病毒病毒(pseudorabies virus,PRV)转移载体pPgG-P1。
- Prourokinase (144 411) Annexin V Chimeras fuse gene was inserted into Bombyx mori baculovirus transfer vector pBacPAK8 and co transfected with lineared DNA of Bm BacPAK6 virus into BmN cells. 将低分子量尿激酶与膜联蛋白 (AnnexinV)的融合基因重组到家蚕杆状病毒转移载体pBacPAK8中 ,获得重组转移载体pBacPAK UKAV ,并与线性化的病毒Bm PAK6DNA共转染家蚕细胞 ,获得重组病毒BacPAK UKAV。
- Additionally, baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non-reproduce vector. 另外,杆状病毒是非复制型载体,能高效表达目的蛋白,其作为基因转移载体在基因治疗中乙显示出良好的应用前景。
- Adenovirus as gene transfer vector is characterized by high transfection efficiency,high titer,good stability in target cells and low pathogenicity,and has been widely used in gene therapy. 腺病毒载体是一种非常有效的转基因载体,具有高效率、低致病性、高滴度及不整合入宿主细胞染色体等优点,广泛应用于基因治疗。
- AAV as gene transfer vector has became a hot spot in gene therapy for eye diseases because of its safety,broad host range,low immunogenicity and long-term expression of therapeutic gene. AAV作为基因转移载体具有安全性好、宿主范围广、免疫原性低和携带的治疗基因长期表达等优势而成为眼病基因治疗研究的热点。
- Adenovirus as gene transfer vector is characterized by high transfection efficiency, high titer, good stability in target cells and low pathogenicity, and has been widely used in gene therapy. 摘要腺病毒载体是一种非常有效的转基因载体,具有高效率、低致病性、高滴度及不整合入宿主细胞染色体等优点,广泛应用于基因治疗。
- acoustic transfer vector ( ATV ) 声学传递向量
- acoustic transfer vector algorithm 声传递向量法
- A transfering vector was constructed by inserted NDV F gene and IBDV VPO gene, under the control of two synthetic promoters ATI P7. 5X20 which were conjugated at opposite direction inserted within the thymidine kinase (TK) gene of FPV 282E4 strain. 将NDV F基因和IBDV VPO基因分别置于人工合成的复合启动子ATI·p7.;5×20的下游,将两个表达单元以复合启动子反向方式连接,插入TK基因中,得到两个复合启动子分别调控两个不同基因的重组转移质粒pVPO-2ATI·p7
- He intends to transfer the property to his son. 他打算把财产转让给儿子。
- This footballer wants a transfer to another team. 这位足球运动员想转到另一个球队踢球。
- The genes coding for hemagglutinin(H) and fusion protein(F) of measles virus were obtained from viral RNA extracted from supernatant of viral culture by RT PCR,and were cloned into baculovirus transfer vectors,respectively. 采用逆转录-多聚酶链反应(RT-PCR)的方法从麻疹病毒Edmonston株基因组中扩增出血凝素H基因和融合蛋白F基因,并利用转移质粒将这两个基因分别重组到杆状病毒多角体蛋白启动子(PH)控制之下,获得重组病毒vBMVH和vBMVF。