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- The supernatant exerted no inhibitory activity. 而其培养上清液无拮抗作用。
- The supernatant was examined by SDS-PAGE protein electrophoresis . 取上清进行SDS-PAGE蛋白质电泳。
- Once precipitated, the sample was centrifuged and the supernatant was removed. 如有沉淀,离心样品,出去上层漂浮物。
- You centrifuge the solution, decant the supernatant, and discard the pellet. 你然后对溶液离心,慢慢倒出上清,弃沉淀。对上清液你加更多的硫酸铵。
- The anisotropic of the supernatant was smaller than that of the bottom layer. 存放还引起粘度下降,估计这与液晶生成会使粘度下降有关。
- You proceed with the purification using the supernatant fraction that contains mostly intact mitochondria. 你继续用含大多为完整的线粒体的上清液纯化。
- In addition, BPF and GM-CSF could also be found in the supernatant collected on 24th day of culture. 培养第24天的上清液中还可检出BPF和GM-CSF活性。
- After enzymatic hydrolysis,the yield of XOS could be up to 84.93% of total carbohydrates of the supernatant. 上清液经木聚糖酶酶解后;低聚木糖的含量可达到上清液总糖的84.;93%25。
- The mIL 12 in the supernatant was detected by ELISA after the pCmIL 12 was transfected into COS 7 cells. p Cm IL - 12在体外转染 COS- 7细胞后 ,经 EL ISA证实有 m IL- 12表达 ,其表达上清能在体外明显增强小鼠 NK细胞活性。
- Many other proteins in the supernatant could interfere the binding of destined protein. 裂解上清杂蛋白浓度较高会干扰目的蛋白的亲和纯化。
- Also, it is likely that there are other viable methods of fractionating the supernatant other than by ultrafiltration. 此外,除了超滤外,可能有其他可行方法把浮于上层的物质分镏。
- You centrifuge the solution, decant the supernatant, and discard the pellet. To the supernatant, which is clearer than the lysate, you add more ammonium sulfate. 你然后对溶液离心,慢慢倒出上清,弃沉淀。对上清液你加更多的硫酸铵。
- Results COS?7 cells transfected with constructed PCIneo?hTPO expressed and secreted hTPO up to 48.28 ng/ml in the supernatant. 应用hTPOcDNA转染的COS-7细胞表达产物对实验性小鼠血小板减少症进行腹腔注射。 结果hTPOcDNA不仅可在COS-7细胞中获得表达,且分泌到细胞培养上清中,最高表达量为48.;28ng/ml。
- FL in the supernatant was detected by ELISA. MTT was adopted to determine the sensitivity of MCF 7/TK FL cells to GCV (ganciclovir). 方法 构建以IRES(内部核糖体进入位点 )相连的TK与FL的真核表达质粒 ,脂质体法转入人乳腺癌细胞株MCF 7中。
- The engineering strain was lysised by ultrasonic wave. Much of MBP-hEra existed in the supernatant of lysate in a soluable form. 表达融合蛋白MBP-hEra菌株经超声波裂解,MBP-hEra大部分以可溶态存在于裂解上清液中。
- The study shows the rhein can appreciably increase the survival rate of acinar cell and lower the levels of LDH,AMY and NAG in the supernatant. 大黄酸可提高相应肿瘤坏死因子浓度下的胰腺细胞的细胞活率,降低培养上清中 LDH、AMY 和 NAG 的水平。
- The supernatant was collected for HCG assay. [Results] Ectopic HCG increased in EAC and JEC with prolonged culture period, especially in JEC. [结果]随着培养时间的延长,两株细胞自分泌的HCG量有所增加,特别JEC株表现更加明显。
- The IL-6 secretion and IL-6 mRNA expression were significantly increased in FLS cultured with the supernatant from U937 cell treated with LPS. LPS刺激的U937细胞培养液上清液可明显增强FLS中IL 6蛋白分泌及mRNA表达 ;
- Both rhCD25MAb and CsA could also decrease the levels of sIL-2R in the supernatant and inhibit the expression of CD25 antigen on T lymphocytes. 虽然CsA和rhCD25MAb均能降低细胞培养上清中的sIL-2R的水平及CD25+/CD3+的比例,但rhCD25MAb的作用明显强于CsA。
- It is this very uneven, so that the supernatant Shenzhen drink team reduced to avoid relegation fight for the team Yunan. 正是这种极不平衡的数据,令深圳上清饮队沦落到了为保级而战的鱼腩球队。