FL in the supernatant was detected by ELISA. MTT was adopted to determine the sensitivity of MCF 7/TK FL cells to GCV (ganciclovir).

• 方法 构建以IRES(内部核糖体进入位点 )相连的TK与FL的真核表达质粒 ,脂质体法转入人乳腺癌细胞株MCF 7中。