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- Analyse the genotype of the target genes using PCR. 聚合酶链反应(PCR)扩增目的基因进行基因型分析。
- The silencing of target gene transcription by unliganded NRs(including AR) may be mediated by these corepressors. 非配体化的核受体(NRs)抑制靶基因的转录可能是通过这些辅阻遏子来介导的。
- Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR. 应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
- The target gene was then inserted into expression vector pET-28a and expressed in E.coli BL21 with IPTG induction. 将mdlA基因克隆到原核表达载体pET-28a中，转化大肠杆菌BL21(DE3)，经诱导后，重组的脂肪酶在宿主菌中的表达量可达菌体总蛋白量的45.;2%25。
- Conclusion: The prokaryotic expression vector with target gene was constructed successfully. 结论:成功构建了带有目的基因的原核表达载体。
- The therapy of antiangiogenesis and target gene offer a broad clinic prospect for lung cancer. 内皮抑素的抗血管生成治疗和血管靶向基因治疗,为治疗肺癌提供了广阔前景。
- Target gene combined with proper vector is transformed in Ecoli cells to amplify. 用适当的载体与目的分子连接后，转入大肠肝菌宿主细胞扩增。
- A previous study has mapped the Ihd gene onto chromosome 10 with SSR markers, but the linked markers were found on one side of the target gene. 先前的研究利用SSR标记已将lhd基因定位在第10染色体上，但只在目标基因一侧找到标记。
- RESULTS: The target gene of recombinant adenovirus plasmid could be amplified by PCR in different passages of HEK293 cells. 用减蛋综合征阳性血清在体外中和重组腺病毒，接种HEK293细胞；
- METHODS: The PSP94 cDNA was obtained from normal prostate tissue, and recombinant plasmid pUC19-PSP94 was constructed. The target gene was identified and sequenced. 方法 :从正常前列腺组织中钓取PSP94cDNA ,构建重组质粒pUC19-PSP94 ,转化后提取目的基因并进行测序。
- The sequence of target gene of recombinant plasmid pcDNA3-ALR constructed in this experiment was in accordance with the sequence of rat ALR cDNA reported. 构建的pcDNA3-ALR重组质粒目的基因与文献报道的大鼠ALR cDNA序列相同。
- With RNA (ribonucleic acid) interference mechanism, scientists have found a new type of anti-AIDS drugs may contribute to the large number of R &D target gene. 借助RNA(核糖核酸)干扰机制，科学家最近找到了可能有助于新型抗艾滋病药物研发的大量目标基因。
- Methods Polymerase chain reaction with restrict enzyme digestion was used to detect the target gene polymorphism in 78 normotension controls and 72 EH patients. 目前认为，高血压是多种遗传因素和环境因素共同作用而引起的复杂性状疾病，在人类遗传易感性的基础上，在环境危险因素的作用下发病。
- Gene therapy is the hot spot currently.This article reviews the progression of anti-neovascularization of retina and chorioid in aspect of animal model,vector and target gene. 基因治疗是目前研究的热点,我们从动物模型、载体、治疗性靶基因的选择等方面对视网膜和脉络膜新生血管治疗的进展进行综述。
- A novel shuttle plasmid which can be replicated in yeast and expressed in mammal cell is required in oral gene therapy with yeast as vector to deliver target gene. 为研究酵母作为载体在口服基因治疗及免疫中的作用,需要一种能够在酵母中复制而在哺乳动物细胞中表达的穿梭载体。
- RNA interference (RNAi) is the process of sequence-specific gene silencing, initiated by double-stranded RNA (dsRNA) that is homologous in sequence to the target gene. 由乙型肝炎病毒（Hepatitis B virus, HBV）感染引起的慢性乙型肝炎是严重危害人类健康的疾病，其主要危害在于HBV感染造成的慢性持续性感染易引起肝硬化，并与肝细胞肝癌（Hepatocellular carcinoma， HCC）的发生密切相关。
- A conditional expression model, in which the target gene is deleted in the presence of a tetracycline derivative, was used to delete the VEGF gene from podocytes. 一个有条件的表达模型用来从足突细胞中删除VEGF基因，在四环素衍生物的出现下靶基因被删除。
- Special RNA molecules stop the synthesis of proteins from a target gene (top); other genes are unaffected (bottom). 特殊的RNA分子正在制止靶基因合成蛋白质(顶);其他的一饮不受影响(底)
- Liver Stem Cells as Vector for Targeted Gene Therapy of Hepatocellular Carcinoma. 肝干细胞作为肝细胞癌靶向基因治疗载体的实验研究
- The RNA copies of viral vectors and mRNA levels of target genes were determined by quantitative real-time RT-PCR. 病毒RNA分子拷贝数和靶基因mRNA水平用实时定量RT-PCR确定。
