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- Inthis study ,Rabies virus cell adapted strain SRV9 , were inoculated in suckling mouse brains to expand anddetected by RT-PCR . The results of TCID50 indicated that there are high titer virus in brains. 本实验将RV SRV9 毒株接种乳鼠,发病后收集鼠脑,经匀浆离心收取上清,RT-PCR 分析,TCID50测定证明,产物中含有高效价狂犬病病毒。
- suckling mouse brain 乳鼠脑
- Methods The changes of NOS neuron of mouse brain in proconditioning to hypoxia were detected by histochemistry method. 方法:采用组化法测定小鼠脑内一氧化氮合酶(NOS)经元在缺氧(次缺氧,4次缺氧)适应中的变化。
- A 50k protein of haemorrhagic fever with renai syndrome virus, puri-fied from infected suckling mouse braln by McAb affinity chromatograpby, was analysed by the Western-blottiug techmc using 18 McAbs specific for the virus. 用18株抗肾综合征出血热(Hemorrhagic fever with renal syndrome,以下简称HFRS)病毒McAb,以Western-blot技术分析了纯化的该病毒50k蛋白。
- With Western blot analysis, the expected 100-kDa RTN3-A1 protein was detected in mouse brain. 用Western杂交的方法，我检测到了预期的100 kDa 的鼠RTN3-A1在脑组织的表过。
- BSG 5 gene was cloned by subtractive different screening as a new gene expressed specifically in mouse brain. BrainSpecificGene 5 (BSG5 )基因是用消减差异筛选的方法克隆到的在小鼠胚胎头部特异表达的新基因。
- The supernatant of 20% brain tissue suspension of suckling mice infected with BA66019 strain of XHF virus, were separated by electrophoresis of SDS-PAGE and bloted in NC membranes by western blot technique . 用新疆出血热病毒原型毒株BA66019感染乳鼠脑的20%25悬液,经差速离心后的上清为抗原进行SDS-PAGE电泳,再用Westernbolt法将凝胶分离的结果印迹到NC膜上。 将6株单克隆抗体小鼠腹水经饱和硫酸铵纯化后,在NC膜上进行结合反应。
- Mobile mouse control screen, the ham, press the left button on the attack, hit as long as the mouse brain will be able to score. 移动鼠标可以控制画面中的火腿,按下左键就可以攻击,只要砸中老鼠的脑袋就可以顺利得分。
- Conclusions Convalescent serum from patient with HFRS may produce protective action to the mouse brain neuron culture cells infected with HTNV. 结论HFRS病人恢复期血清具有一定的中和病毒保护培养的神经元作用。
- Histopathological examination showed the appearance of MCMV-specific intracytoplasmic inclusion bodies in neuron cells of the mouse brain. 结论 小鼠巨细胞病毒模型的建立为抗-CMV有效药物的筛选以及对CMV感染的预防、治疗提供了资料。
- Zeng Z, Zhang Z, Yu H, Corbley MJ, Tang Z, Tong T.Mitochondrial DNA deletions are associated with ischemia and aging in Balb/c mouse brain. 曾昭惠,段建明,张宗玉,童坦君;鼠毛及脑线粒体DNA片段缺失与增龄的关系;中国生物化学与分子生物学报;1999;15(2):320
- Positive immunohistochemical staining for SARS-CoV was detected in tissues of suckling mice and Vero E6 cells with use of sera from SARS patients. 接种SARS患者标本的发病乳鼠组织以及病变的Vero E6细胞与SARS患者血清发生免疫学了反应。
- The damage of mouse brain after microinjection was examined by flow cytometry using PI stain or Annexin V plus PI stain. 无论是从脑干或海马迴感染，鼠脑的脑干皆有超过百分之十的损伤死亡；
- Either Ae.al,bopietus or Ae aegypti for 5th to 6th day after infection were capable of transmitting CHIK virus by biteto suckling mice. 无论白纹伊蚊或埃及伊蚊，感染后第5-6天即可通过吸血将病毒传播给乳鼠，至第8-13天，传播率可高达55．55%25-100%25。
- They then went on to show that injecting the VGF gene product into mouse brain produces an antidepressant response in the animals. 接着下来，他们将VGF基因产物注入老鼠的大脑当中，并且观察到它在动物中的抗抑郁反应。
- Methods Cytopathic effect (CPE) and pathogenicity were observed in C6/36 cells and in suckling mice inoculated intracerebrally with the WNV or JEV, respectively. 方法通过给C6/36细胞和乳鼠接种WNV或JEV,观察两种病毒的CPE和致病性;
- We describe here an anatomically comprehensive digital atlas containing the expression_r patterns of 20,000 genes in the adult mouse brain. 在这里我们描绘一种解剖学上的综合性数字地图包含成年小鼠大脑20000个基因的表达模式。
- Objective To study the effects of extremely low frequency electromagnetic field(ELF EMF) and its combination with lead on the antioxidant system in mouse brain and liver tissues. 目的 研究极低频电磁场及其与铅的联合作用对小鼠脑和肝脏抗氧化系统的影响。
- Meanwhile,the mouse brain tissue homogenate colony count (CFU) was observed and the results were compared with that of the immunohistochemical examination. 同时观测小鼠脑组织匀浆后菌落计数(CFU),并与免疫组织化学结果进行对照分析。
- Meanwhile, D2V-HN89 was most close to D2V-Jamaica and D2V-Brazil90. The cytopathogenic effect of D2V-HN89 and D2V-NGC were similar but the neural virulence of D2V-HN89 in suckling mice was much weaker than that of D2V-NGC. D2V HN89株的细胞病变效应与D2V NGC株相同 ,但对乳小白鼠神经毒力较弱。
