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- The segments amplified with 3 cpDNA and 3 mtDNA universal primers were digested by 15 restriction endonucleases. 3个叶绿体和3个线粒体通用引物扩增出的片段，用15种限制性内切酶对其进行酶切。
- Methods: Cutting the pGEM-7Zf -DAF plasmid with restriction endonucleases,and obtaining the recombinant human DAF gene which containing ICAM-2 promotor fragment. 方法:双酶切本室已构建质粒pGEM -7Zf-DAF ;得到含人ICAM -2启动子及DAFcDNA序列的插入片段 (3.;7Kb) ;
- SeMNPV; Chinese strain; isolation and identification; bioassay; restrict endonucleases analysis; polyhedrin; chitinase; alignment of homology. 01甜菜夜蛾核多角体病毒;中国分离株;分离鉴定;生物测定;限制性内切酶分析;多角体;几丁质酶;同源性比较。
- Some restriction endonucleases , cleave DNA at random, but a particular group of enzymes, known as class II restriction endonuclearses, cleave DNA at special sits. 一些限制性内切酶的切割DNA的作用是随机的，但是相当一部分的内切酶，如限制性内切酶II，就是在特定的位置切割DNA。
- The plasmid PE which harbored the whole hexon-encoding gene of egg drop syndrome virus(EDSV) was identified and the hexon protein gene was obtained from it by restriction endonucleases. 对其它腺病毒的研究表明,对六邻体蛋白基因的克隆、表达、纯化将有利于腺病毒的免疫学研究,纯化了的蛋白进一步进行活性鉴定,可用作抗原,用于动物免疫,并检测其所产生的抗体。
- Its genome was composed of over 14 DNA molecules,which were different in molecular size and abundance. Molecular size of the genome was approximate 108 kbp calculated by patterns from six restrict endonucleases digestion. 琼脂糖凝胶电泳显示MmPDV基因组至少由大小不同、丰度不等的 14个DNA分子组成 ,用 6种内切酶 (EcoRI,HindIII,BssHII,PstI,BamHI,BglI)酶切MmPDV基因组后 ,估算出MmPDV基因组大小约为 10 8kb。
- A ,sad sequence ;B ,restriction endonuclease cutting sites of sad. 段,与序列分析结果相一致(图略)。
- The normal size of the fragments from the plasmids cut by endonucleases: The results of restriction endonuclease analysis and agar electrophoresis indicated that size of plasmids pMSCV, CED-4pMSCV, pGAG-POL and pVSV-G was normal. 质粒DNA酶切片段大小正常：酶切质粒、电泳结果表明，pVSV-G, pGAG-POL, pMSCV,CED-4pMSCV 质粒DNA大小正常；
- restriction endonucleases from Haemophilus influenzae 流感嗜血杆菌释放的限制核酸内切酶
- Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid. 方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
- Mimics of both types of BCR ABL cDNA were achieved and the validity was verified with restriction endonuclease. 经酶切分析证明 ,两型BCR ABLmRNA均可通过此方法得到相应的cDNA参照物。
- Results The result of restriction endonuclease digestion was accordance with the anticipated objective strap size . 结果 酶切结果与预期目的条带大小相符；
- PCR amplification and restriction endonuclease digestion was used to identify deleted DNA fragments. 应用PCR技术与限制性内切酶酶切相结合的方法鉴定缺失子。
- Method The two clones of OSCP gene were modified by restriction endonuclease and recombined by T_4DNA ligase. 方法通过限制性核酸内切酶将发生有义突变的两个OSCP基因的DNA克隆进行改造,并进行基因重组。
- The 344C/T polymorphism of CYP11B2 gene was monitored by PCR and HaeIII restriction endonuclease digestion methods. 应用多聚酶链式反应(PCR)、限制性内切酶方法检测CYP11B2基因的多态性分布。
- After the PCR amplicons of SEE strain sere eleaved by restriction endonuclease EcoRV.electrophofretic analysis showed two 251 and 415bp DNA fragments. SEE菌株的扩增产物经EcoRV酶切能产生251和415bp两个片段。
- A portion of Ligation products were identified by BamHI and Hin-dIII restriction endonuclease and was named pUC - B2 - AR. 2·PMCX－p。 －AR反义表达载体的酶切鉴定，其结果与理论设计完全相符。
- The recombinant plasmid pAd-K14-E6/E7-polA was successfully constructed and confirmed by restriction endonuclease digestion and sequencing. 通过同源重组的方法构建了腺病毒pAd-K14-E6/E7-polA载体,经酶切和测序鉴定该质粒构建成功。
- Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful. 结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。
- The recombinant plasmid PsecTaq2A_AMG was analyzed by restriction endonuclease mapping and DNA sequencing. The results of sequencing were consistent with those from GenBank. 重组克隆PsecTaq2A_AMG酶谱分析与预期结果一致 ,序列测定结果与GenBank中的人釉原蛋白序列完全一致。
