Methods: Cutting the pGEM-7Zf -DAF plasmid with restriction endonucleases,and obtaining the recombinant human DAF gene which containing ICAM-2 promotor fragment.

• 方法:双酶切本室已构建质粒pGEM -7Zf-DAF ；得到含人ICAM -2启动子及DAFcDNA序列的插入片段 (3.；7Kb) ;