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- Methods The 245 bp fragment of human MTB (H 37 Rv) gene sequence was repeated multiple times through PCR, and established reconstructive plasmid. 方法 通过PCR特异扩增人型结核分枝杆菌 (H37Rv)IS986基因序列 2 45bp片段 ,构建含该特异片段的重组质粒 ,以该重组质粒DNA为模板 ,用PCR扩增IS986产物制备长臂光敏生物素标记的基因探针 ,进行探针特异性检测。
- The relative potency of supernatant increased by 216% and had stable inheritance,after LC47 was treated with repeated multiple mutation with ultraviolet radiation(UV) and diethyl sulphate(DES). 经紫外线照射(UV)和硫酸二乙酯(DES)多轮复合诱变,LC47发酵液相对效价较未诱变前提高了216%25,且具有良好的传代稳定性。
- I shall have to report you for repeated lateness. 你老是迟到,我可要告你了。
- He made a resolution never to repeat the act. 他决心不再采取那种行动。
- I was embittered by repeated failures. 我因屡次失败而苦恼。
- He repeated her statement word for word. 他一字不差地重复她的话。
- Or you can say history repeat itself. 或者说也可以说,历史经常重演。
- He repeated several times that he was busy. 他一再说他很忙。
- The sweater had shrunk after repeated washing(s). 这件套头毛衣洗过多次已缩水了。
- My son began to study multiplication when he was six. 我儿子六岁的时候开始学习乘法。
- The repeated failure has made him lose courage. 多次失败使他失去了勇气。
- The multiplication of students has made our school too small. 学生的增加使学校显得拥挤。
- They made me repeat/I was made to repeat the story. 他们逼我又把那事讲了一遍。
- He was embittered by repeated failures. 他因屡遭失败而痛苦。
- Our teacher used to drum our multiplication table into us. 我们老师过去老是让我们反复背诵乘法表。
- He was frustrated by repeated failures. 他因一再失败而灰心丧气。
- His plans have suffered repeated delays. 他的计划屡遭拖延。
- Our teacher used to drum our multiplication tables into us. 我们老师过去老是让我们反覆背诵乘法表。
- He repeated the conversation word for word. 他原原本本地复述了那段谈话。
- She repeated what I had told her. 她把我对她说的话讲给别人听了。