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- METHODS: VEGF protein production from ECV304 cells was determined by enzyme- linked immunosorbent assay (ELBA). 方法:在人脐静脉内皮细胞株ECV04培养基中加入不同浓度的LPC,培养不同时间,用基础酶联免疫吸附实验(ELISA)检测各组EC条件培养基中VEGF蛋白含量;
- Real-time RT-PCR and western blotting were used to assess the relatie adiponectin receptor mRNA expression and protein production, respectiely. 用荧光定量逆转录PCR和免疫蛋白印迹评估了相关脂联素受体各自的基因表达和蛋白产生。
- The expression vector of PVS CP gene is constructed, a 58kDa fusion protein product is expressed in E. 构建了含PVS CP基因的融合蛋白原核表达载体，并在大肠杆菌(E.;coli
- Gunter still points out, the different point of protein and carbohydrate is protein production muscle, and carbohydrate provides energy for muscle. 我觉得无论你是初学者还是有一定经验的练习者，都应该吃点红肉，只不过要吃瘦肉。”
- The NF1 tumor suppressor encodes a protein product neurofibromin,which functions as a negative regulator of RAS. NF1为肿瘤抑制基因,是RAS的负向调节器。
- In EM, this material will turn out to be abundant rough endoplasmic reticulum, which is associated with protein production. 电镜下这些物质是大量的粗面内质网，它们与蛋白质合成有关。
- Its protein product was found to also suppress metastasis of melanoma cells and murine mammary carcinoma cells. 它所编码的蛋白还可以抑制黑素瘤细胞和小鼠乳腺癌细胞的转移。
- Essentially the ribo-regulator enables scientists to dictate protein production, with close to 100 percent accuracy and efficiency. 这种RNA核糖调控子使科学家在控制蛋白质的产生时，效率与精确度几乎可达100%25。
- The protein product of the hemochromatosis (HFE) gene modulates uptake of iron and dialent cations from pulmonary sources and reduces their toxicity. 血色病基因的蛋白产物调解肺源Fe和二价阳离子的摄取，并能降低其毒性。
- Hofbauer LG,Khosla S,Dunstan CR,et al.Estrogen stimulates gene expression and protein production of osteoprotegerin in human osteoblastic cells[J].Endocrinology,1999,140(9):4367. 关美萍.;雌激素和雌激素受体基因多态性与骨代谢异常的关系[J]
- Of these, the protein products of the Aux/IAA genes are the best characterized. 在这三类基因中，Aux/IAA基因家族的研究相对较多。
- The aims of this thesis are to develop new serum-free media for recombinant Chinese hamster ovary (CHO) cells and to increase the recombinant protein production of CHO cells. 本论文的主要目标为开发适合中国仓鼠卵巢细胞(CHO)的无血清培养基与提高CHO之重组蛋白质产量。
- Creamy fish protein (CFP) is a kind of fish protein product produced by the method of enzymatic hydrolysis using low value fish as material. 乳状鱼蛋白是以鱼类为原料采用酶水解方法制作的一种鱼蛋白制品。
- The use of plants as an expression system for recombinant protein production would be at least as economical as traditional industrial facilities (by fermentation processes or bioreactor systems). 利用植物作为重组蛋白生产的表达系统至少比传统的工业制造方法（利用发酵过程和生物反应系统）要便宜。
- In the ease of same amount of fertilizerapplication to the same varieties,improving moisture condition can improvegrain yields aed N uptakes by wheat plants and raise protein production butnot raise the protein contents in grains. 同一品种在施肥量相同时,改善水分条件可以提高籽粒产量,提高对氮素的吸收量,提高蛋白质产量,而不能提高籽粒蛋白质含量。
- Mammalian cells are the ideal host cells for expressing recom binant protein products. 摘要 哺乳动物细胞是表达蛋白类生物产品的理想宿主细胞。
- Baculovirus/insect cell system has been widely used for recombinant protein production, but traditional system eventually resulted in cell lysis, so that the expressed recombinant protein was lost into medium. 摘要:杆状病毒/昆虫细胞系统已经被广泛的应用在重组蛋白质的生产上，但传统的杆状病毒感染后会造成细胞溶裂，而使得表现出的重组蛋白质流失到培养基中。
- Methods:The full-length gene of NY-ESO-1 was generated by gene splicing method and the recombinant expression vector NY-ESO-1-pET-28a (+) was constructed. E. coli BL21 (DE3) bearing the plasmid was induced with IPTG for protein production. 方法:通过全基因拼接获得NY-ESO-1基因,构建重组表达载体NY-ESO-1-pET28a(+),在大肠杆菌BL21(DE3)中利用IPTG诱导获得表达,利用单克隆抗体进行Western印迹鉴定,通过Ni柱亲和纯化获得纯化蛋白。
- A crucial aim upon the completion of the human genome is the verification and functional annotation of all predicted genes and their protein products. 人类基因组项目完成的一个根本目的是对所有预测的基因及其蛋白产物的辨识与功能注释。
- The lung tissue was collected 1,2 and 6 hours after the injection of LPS. The protein production of CINC was measured by immunohistochemical,while the mRNA expression of CINC was measured by RT-PCR. 用免疫组织化学法和半定量RT-PCR分别检测CINC蛋白及其mRNA在造模后1、2和6 h肺组织中的表达变化。
