METHODS: VEGF protein production from ECV304 cells was determined by enzyme- linked immunosorbent assay (ELBA).

• 方法:在人脐静脉内皮细胞株ECV04培养基中加入不同浓度的LPC,培养不同时间,用基础酶联免疫吸附实验(ELISA)检测各组EC条件培养基中VEGF蛋白含量;