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- prokaryotic expression vection 原核表达载体
- Objective:To clone,prokaryotic express and purify APOBEC3G protein in vitro. 目的:原核表达重组APOBEC3G蛋白,为其功能及免疫原性研究奠定基础。
- Studies on Prokaryotic Expression of Plant Anti-freeze and Salt Tolerance Genes. 盐基因原核表达研究。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective: 1.Subclone B7.2(IgV+C),IgV and IgC domains and construct prokaryotic expression system of them. 2. 目 的： 1．亚克隆B7.;2分子的胞外(IgV+C);IgV及IgC各区段并构建其原核表达载体。
- Conclusion: The prokaryotic expression vector with target gene was constructed successfully. 结论:成功构建了带有目的基因的原核表达载体。
- Objective To clone the flagellar biosynthesis genes flhA,flhB_2 and fliR of Leptospira interrogans and construct their prokaryotic expression system. 目的克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR,并构建其原核表达载体。
- Objective: To obtain the recombinant augiogenin (Ang) protein by means of prokaryotic expression system and investigate its bioactivity. 目的:利用原核表达系统获得重组血管生成素 (Ang)并研究其生物学活性。
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
- Full-length or truncated cDNA was subcloned into prokaryotic expression vector pET30a and expression induced in E. coli BL21(DE3). 全长的或C末端截短的鲨烯合酶cDNA被克隆进原核表达载体pET30a并在大肠杆菌BL21(DE3)中诱导表达。
- Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根据GenBank S.;suis2epf基因序列设计引物;克隆ZYH24株epf基因片段并进行序列分析;
- Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物，并构建了pGEX-4T-1-BLY原核表达载体。 经BamHI和EcoRI酶切及质粒PCR鉴定，证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上，为进一步研究其诱导表达条件及生物学功能奠定了基础。
- Prokaryotic Expression of the Vacuolar Ca~(2+)/H~+ Antiporter SsCAX1 N-termi-nal and Its Polyclonal Antibody Preparation of Suaeda salsa L. 盐地碱蓬液泡膜Ca~(2+)/H~+逆转运蛋白SsCAX1 N末端原核表达和多克隆抗体的制备
- Prokaryotic expression of structural domains of the Newcastle disease virus fusion protein and analyses of their antigenic epitopes. 新城疫病毒F蛋白结构域基因原核表达与抗原表位分析
- The choEW was inserted into prokaryotic expression vector pET-His, and the resulting recombinant plasmid pETW was used to transform E. colt BL21(DE3)plysS. 将choEW插入到原核表达载体pET-His中,构建出重组质粒pETW,并转化Escherichia coli BL21(DE3)plysS获得工程菌。
- We reconstructed the gene of human scFv (VL-VH) and prepared scFv(VL-VH) and scFv (VH-VL) antibody fragments by prokaryotic expression system PET22b(+)/BL21(DE3). 对筛选获得的人源抗G5-24 scFv(VL-VH)基因进行了重构，将它们克隆入表达载体pET22b(+)中，转化大肠杆菌BL21(DE3) ，IPTG诱导表达；
- High efficient expression of Pro-X lays the foundation for serum assay of PBC patient by prokaryotic expression of dihydrolipoamide acetyltransferase compounds . 获得Pro-X蛋白的原核高效表达,为利用原核表达的丙酮酸复合物对PBC患者进行血清学检测进一步奠定了基础。
- The study aimed to construct the prokaryotic expression vectors carrying MTB esat6 and lhp and lhp-esat6 fusion genes amplified by PCR, and express them in E. coli respectively. 本研究通过体外扩增MTB esat6、lhp及lhp-esat6融合基因，构建原核表达载体并在E.
- Inducible prokaryotic expression vector pET-Nus-STK11(with Nus fusion tag) was constructed with pET-44a(+) and the cDNA of STK11 gene cloned in our lab. 利用本室克隆的人STK11 cDNA和原核表达载体pET-44a(+)构建带有Nus融合标签的诱导型表达载体pET-Nus-STK11,在不同的大肠杆菌宿主中诱导表达。
- Methods:(CTP)_4coding gene from pBSMR-(CTP)_4 was cloned into prokaryotic expression vector pET-28a(+) and the expression plasmid pET-28a(+)- (CTP)_4was obtained. 方法:将pBSMR-(CTP)_4中的(CTP)_4基因片断克隆入表达载体pET-28a(+),得到表达质粒pET-28a(+)-(CTP)_4。
