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- The prokaryote expression system is used to express firstly functional B7.2 peptide fragments and demonstrate that the expressed products has similar costimulatory biological activity. 3 . 2．首次用原核系统表达出具有生物活性的共刺激分子B7.;2。
- Objective:To clone,prokaryotic express and purify APOBEC3G protein in vitro. 目的:原核表达重组APOBEC3G蛋白,为其功能及免疫原性研究奠定基础。
- Studies on Prokaryotic Expression of Plant Anti-freeze and Salt Tolerance Genes. 盐基因原核表达研究。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective: 1.Subclone B7.2(IgV+C),IgV and IgC domains and construct prokaryotic expression system of them. 2. 目 的： 1．亚克隆B7.;2分子的胞外(IgV+C);IgV及IgC各区段并构建其原核表达载体。
- Conclusion: The prokaryotic expression vector with target gene was constructed successfully. 结论:成功构建了带有目的基因的原核表达载体。
- Objective To clone the flagellar biosynthesis genes flhA,flhB_2 and fliR of Leptospira interrogans and construct their prokaryotic expression system. 目的克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR,并构建其原核表达载体。
- Objective: To obtain the recombinant augiogenin (Ang) protein by means of prokaryotic expression system and investigate its bioactivity. 目的:利用原核表达系统获得重组血管生成素 (Ang)并研究其生物学活性。
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
- Full-length or truncated cDNA was subcloned into prokaryotic expression vector pET30a and expression induced in E. coli BL21(DE3). 全长的或C末端截短的鲨烯合酶cDNA被克隆进原核表达载体pET30a并在大肠杆菌BL21(DE3)中诱导表达。
- Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根据GenBank S.;suis2epf基因序列设计引物;克隆ZYH24株epf基因片段并进行序列分析;
- Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物，并构建了pGEX-4T-1-BLY原核表达载体。 经BamHI和EcoRI酶切及质粒PCR鉴定，证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上，为进一步研究其诱导表达条件及生物学功能奠定了基础。
- Prokaryotic Expression of the Vacuolar Ca~(2+)/H~+ Antiporter SsCAX1 N-termi-nal and Its Polyclonal Antibody Preparation of Suaeda salsa L. 盐地碱蓬液泡膜Ca~(2+)/H~+逆转运蛋白SsCAX1 N末端原核表达和多克隆抗体的制备
- Prokaryotic expression of structural domains of the Newcastle disease virus fusion protein and analyses of their antigenic epitopes. 新城疫病毒F蛋白结构域基因原核表达与抗原表位分析
- PROKARYOTE EXPRESSION AND WESTERN ANALYSIS OF BcpLH GENE OF CHINESE CABBAGE 大白菜BcpLH基因的原核表达及其蛋白产物的Western分析
- An expression of pain flitted across her face. 她脸上闪过一种痛苦的表情。
- He always shows a doleful expression. 他总是露出忧郁的表情。
- The choEW was inserted into prokaryotic expression vector pET-His, and the resulting recombinant plasmid pETW was used to transform E. colt BL21(DE3)plysS. 将choEW插入到原核表达载体pET-His中,构建出重组质粒pETW,并转化Escherichia coli BL21(DE3)plysS获得工程菌。
- I can think of no greater expression of defeatism. 这是我听到的最悲观的失败主义论调了。
- A constant, variable, function name, or expression. 一种常数、变量、函数名或表达式。
