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- AL01 library contained 23 650 positive clones and the av-erage foreign DNA fragments were about 3.2 kb. AL01文库包含23650个克隆;随机插入载体的外源DNA 片段平均大小为3.;2Kb 左右;DNA 文库的总容量为75
- The obtained 30 positive clones were all sequenced, and analyzed by BLAST (basic local alignment search tool). 将得到的30个阳性克隆全部进行测序,并进行BLAST同源序列比对分析。
- Conclusion The identification of positive clones provides a possible way for the development of toxoplasmosis vaccine. 结论阳性克隆的筛选和鉴定为抗弓形虫病疫苗的研制提供又一途径。
- SPV Vp2 protein was successfully expressed by the positive clones under the induction of IPTG. (2)首先用大肠杆菌表达和部分纯化的SPV VP2融合蛋白建立的Western印迹分析检测了猴子暴露者中的血清SPV抗体,同时用检测人细小病毒B19 IgG抗体的商品试剂检测细小病毒抗体以排除可能存在的B19的交叉抗体反应。
- By sequence alignment, 109 special microsatellite positive clones were confirmed finally, which contained 179 microsatellite DNA domains. 序列比对后最终获得109个具有特异微卫星序列的阳性克隆,其中包含179个微卫星DNA结构域。
- Results: After 4 rounds of panning,20 positive clones were obtained and 18 clones were found to be able to bind keratin antigen specifically. 结果: 经过4轮筛选,获得20个能与角蛋白结合的阳性克隆,其中可产生特异性抗角蛋白抗体的克隆18个。
- Thin recombinant with the cDNA ~crt fragment was released frc the positive clone and was di ?z; tcJ with EcoRI; the eDNA fra, ent was 2kb in length. 释放质粒后,进行酶切鉴定,该阳性克隆cDNA插入片段的大小为2kb左右。
- Methods Newborn larvae(NBL) cDNA library of Trichinella spiralis was screened using the infected swine serum and positive clones were sequenced and analysed. 方法 以旋毛虫感染猪血清为抗体探针 ,对旋毛虫新生幼虫cDNA文库进行免疫筛选 ,将获得的阳性克隆进行测序及分析。
- The library was screened by the RGA probe of Hvrgak4. Four positive clones were obtained among 1.2 X 106 clones in the library through two rounds southern hybridization. 从cDNA文库的1.;2×10~6个克隆中经两次筛选获得四个阳性克隆:kong1插入片段2
- By screening the work (Apis cerana cerana) heads cDNA library, using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. 以东方蜜蜂(Apis cerana)mrjp3基因部分片断为探针筛选中华蜜蜂(Apis cerana cerana,简称中蜂)8日龄工蜂头部cDNA文库,得到120个阳性克隆。
- By screening the worker(Apis cerana cerana)heads cDNA library, using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. 以东方蜜蜂(Apis cerana)mrjp3 基因部分片段为探针筛选中华蜜蜂(Apis cerana cerana,简称中蜂)8 日龄工蜂头部 cDNA 文库,得到 120 个阳性克隆。
- The HCV-specific phage antibodies were highly enriched after five rounds of biopanning against HCV NS4 mosaic antigen and positive clones were detected upto 96% by ELISA. 经5轮亲和选择可使特异性噬菌体抗体得到高度富集,抗HCV噬菌体抗体阳性克隆达96%25。
- Blast analysis displayed one of the Northern blot positive clone is homologous with autotaxin gene, and another is homologous with calcyclin gene. 对这13个克隆进行测序和Blast同源序列分析表明,其中一个Northern杂交阳性克隆与autotaxin基因同源,另一个与钙周期蛋白基因同源。
- Methods:Screening peptide library with EAN serum. Identified positive clone by ELISA and compared similarity between positive clone and LPS of Campylobacter jejuni. 方法 :采用噬菌体呈现技术对 8个EAN模型血清抗体进行肽库筛选 ,并将获得的克隆用ELISA方法进行阳性鉴定 ,以及将阳性克隆与空肠弯曲菌LPS相似性进行比较。
- The positive clones were screened by G418.The proliferation,migration,and invasion of MDA-MB-231 cells before and after transfection were assessed by CFSE-flow cytometry,monolayer wound healing assay,and soft agar colony formation test. 应用CFSE-流式细胞仪检测,单层伤口愈合实验,软琼脂克隆形成实验等方法观察了MDA-MB-231细胞转染前后,细胞体外增殖及侵袭能力等指标的变化。
- The recombinant plasmid containing ATF-PAI2CD fused gene, pZWY-ATF-PAI2CD, was transferred to the competent cells of yeast strain GS115. After the phenotype selection, the positive clones were induced with methanol to express ATF-PAI2CD. 该质粒转化毕赤酵母菌GS115 ;用G4 18 YPD平板筛选高拷贝转化子 ;然后用甲醇诱导表达 .;工程菌用摇瓶发酵;表达产物ATF PAI2CD占培养液中总蛋白 5 0%25以上
- The recombinants with the cDNA insert fragment were released from the positive clones and were digested with EcoR I , The cDNA fragment were 2.1kb and 1.5kb in lycB and IPI positive clones respectively. 释放质粒后,进行酶切鉴定,LycB阳性克隆cDNA插入片段的大小为2.;1kb左右,IPI阳性克降cDNA插入片段的大小约为1
- There is positive proof that he did it. 有确切的证据证明他做了此事。
- Sequening of the positive clones show that there are 3 significative molecules interacting with mPem: Psap, Brpfl and CDC37.Brpfl ( bromodomain and PHD finger containing 1) includes one bromodomain,two PHD finger and one PWWP motif. Brpf1基因(bromodomain and PHD finger containing 1)含有1个保守的溴结构域(bromodomain)、两个PHD锌指结构和1个PWWP模序。
- By sequencing the positive clones directly, three novel genes (Genbank accession number: AF179285, AF 170307 and AF194971 ), with transcription sizes of 2. 1 Kb, 1. 1Kb and 1. 4 Kb respectively, were isolated successfully. 结果:克隆了转录本为2.;2Kb、1