The recombinant plasmid containing ATF-PAI2CD fused gene, pZWY-ATF-PAI2CD, was transferred to the competent cells of yeast strain GS115. After the phenotype selection, the positive clones were induced with methanol to express ATF-PAI2CD.

 
  • 该质粒转化毕赤酵母菌GS115 ;用G4 18 YPD平板筛选高拷贝转化子 ;然后用甲醇诱导表达 .;工程菌用摇瓶发酵;表达产物ATF PAI2CD占培养液中总蛋白 5 0%25以上
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