- The pCR3. I vector has CMB promoter that is highly efficient for expression of target protein. The positive plasrnid was extracted using plasmid quick extraction kit. Liposorne was used as intermediate to transfect the pCR3.l-hTERT plasmid into DK cell.
- The Grb-ast8 concatemer was cloned into pET22b expression vector. The result of sequencing confirmed the reading frame of the recombinant plasrnid was correct. Then, the recombinant plasmid was transformed into expression host E.
- Keywords Gentamicin;Gentamicin resistant plasrnid;Phage;
- Recombinant plasrnid