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- The recombinant plasmid pBV220-Arr was construted successfully and the target protein Arresten can express in E. 成功构建Arresten基因重组质粒pBV220-Arr;并可在E.
- The result showed the target protein was successful expressed as a SLH-Ap36 fusion protein. 结果表明,目标蛋白以融合蛋白的形式成功表达。
- After purification, the target protein further renaturation 2 - BMP via degeneration dissolving, load dialysis bag. 纯化后的的目的蛋白进一步复性,BMP-2经变性液溶解,装入透析袋内。
- The reconstructed vector NY-ESO-1-pET-28 could express target protein stably in the soluble fraction of bacterial extract. 构建的原核表达载体NY-ESO-1-pET28a(+)可稳定地可溶性表达NY-ESO-1蛋白;
- The 14-3-3 proteins interact with targeting proteins or two different domains in one targeting protein by phosphoserine /phosphothreonine motifs. 7种不同亚型的高度保守的14-3-3蛋白与人类细胞中多种不同的信号通路有密切的关系。
- Some phages showing low, medium, high specificity to each target protein, lysozyme, insulin and RNase A, had been selected respectively for sequencing. 利用新建立的交替洗脱法,使用其它目标蛋白质对噬菌体展示肽库进行筛选,又获得了对胰岛素和核糖核酸酶A具有高亲和力的噬菌体。
- By expression vector pHHSM002, tpsl gene also expressed efficiently in E. coli BL21(DE3)PlysS ,the target protein amount to 17.8% in cell. 以pHHSM002为表达载体,tps1基因在大肠杆菌BL21中也得到了高效表达,表达的目的蛋白占菌体总蛋白的17.;8%25。
- Pharmacophore hypothesis(PH) is fundamental to de novo design of peptide ligand based on the structure of target protein. 在基于靶蛋白结构的多肽分子设计中,药效假说是从头设计方法的基本前提。
- Other uses of microarrays include the screening of large numbers of molecules for their capacity to bind a particular target protein. 其它微点阵的用途包括从大量分子中筛选出能结合特定靶蛋白的分子。
- SUMO protease can cut SUMO fusion protein expressed by fusion expression system without any amino acid residues left on target protein thus become a hot topic in this field. SUMO蛋白酶对SUMO融合表达系统表达的重组蛋白进行切割时没有多余氨基酸残留,因此成为蛋白切割工具的热点。
- Using different density urea to isolate the inclusion body, then purify the target protein through the Ni-NTA resin, analyzed with SDS-PAGE found the purity attained over 90%. 用尿素梯度分离包涵体,确定1 M尿素洗涤包涵体为分离MP-GFP的最优条件。经Ni-NTA树脂纯化目标蛋白,SDS-PAGE电泳分析其纯度达到90%25以上。
- A new nuclear power station is on the stocks here. 这座新的核电站在建造之中。
- This old man is a famous nuclear physicist. 这位老人是一位著名的核物理学家。
- Plans for nuclear plants have been declassified. 建造核装备的计划已不再保密。
- They decided to establish a nuclear power station. 他们决定修建一所核电站。
- SDS-PAGE results showed that there was a clear target protein band in Mut+ recombinant supernatant after 48 hours of culturing, while a faint band only in Muts recombinant after 72 hours. 结果表明,诱导培养48小时后,Mut~+重组菌株表达产物在SDS-PAGE胶上显现出清晰的目的蛋白带,而Mut~s重组菌株培养72小时才能显示微弱的目的带;
- Nuclear energy remains a double-headed creature. 核能依然是一种有利也有弊的能原。
- Nuclear physics is a closed book to me. 核物理学是我完全不懂的一门学科。
- Nuclear physics is a closed book to most of us. 我们大多数人对原子核物理学一窍不通。