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- northern blotting RNA 印迹法
- northern blot RNA 印迹
- Northern blot -- An electroblotting method in which RNA is transferred to a filter and detected by hybridization to32 P-labelled RNA or DNA. 北方转渍法--一种电子转印方法,将RNA转移至滤纸上,加上要试验的放射性标志RNA或DNA,以进行杂合,以自动显影技术观察
- Animals were killed at day 3,7,14and 28 after BLM administration. The total RNA was extracted from the lung tissue. The expressions of SPsmRNA were analyzed with Northern blot. 方法气管内灌注博莱霉素A5,复制肺纤维化模型,分别于3、7、14和28天处死动物,提取肺组织总RNA,进行Northern杂交。
- Methods:Messenger RNA expressions of nm23-H1 and nm23-H2 genewere investigated in 12 cases of human bladder transitional cell tumor and 6 cases of human normalbladder tissue by using the Northern blot techniques. 方法:应用Northernblot技术分析12例膀胱移行细胞癌和6例肉眼未见异常的癌旁膀胱粘膜nm23-H1和nm23-H2基因的表达。
- Northern blotting was used to evaluate the mRNA expression of WISP3, a member of the CCN family, mRNA in the OBs. 同时采用Northern杂交检测成骨细胞的WISP3表达情况。
- Northern blot showed that PBP-Harm and GOBP2-Harm are specifically expressed in the antenna of H.armigera. Northern杂交结果表明:PBP-Harm和GOBP2-Harm都在棉铃虫触角中特异性表达。
- The mRNA expression of ubiquitin in rat EDL muscle was determined by northern blot analysis. 应用northernblot方法测定伸指长肌组织泛素mRNA的表达变化 ,分析骨骼肌蛋白降解与泛素mRNA表达的关系。
- In this study the authors detected both nm23 H 1 and nm23 H 2 mRNA levels in 52 tissues samples of patients with carcinoma of buccal mucosa (CBM) by Northern blotting method. 通过Northern斑点杂交技术研究52例颊癌及相关组织中肿瘤转移抑制基因nm23-H1和nm23-H2mRNA的表达。
- PAI-1 protein secretion by mesangial cells was measured by Wes tern blot and PAI-1 mRNA by both RT-PCR and Northern blot. RT-PCR和Northern杂交检测PAI-1mRNA表达;
- With RT-PCR and Northern blot methods, the expression patterns of mNTKL-BPl were observed in the normal tissues of mouse. 用RT-PCR和Northern blot方法观察到mNTKL-BP1基因在小鼠的正常组织中均有表达。
- Transgenic potato plants 1M, 2M, 3M, 5M, 10M, llM provided by laboratory were analyzed by Southern blot and Northern blot. ELISA检测转基因马铃薯病毒抗性数据显示,温室条件下,3个转基因马铃薯株系(2M、3M、11M)具有病毒抗性,3个株系(1M、5M、10M)感病;
- Blast analysis displayed one of the Northern blot positive clone is homologous with autotaxin gene, and another is homologous with calcyclin gene. 对这13个克隆进行测序和Blast同源序列分析表明,其中一个Northern杂交阳性克隆与autotaxin基因同源,另一个与钙周期蛋白基因同源。
- Expression of messenger endothelin 1 (ET 1) RNAs and its receptor subunits (ETA,ETB)were studied by northern blot hybridization in rat kidney following renal ischemia reperfusion. 为了探究内皮素?1(ET?1)对肾功能的影响和作用方式,采用斑点杂交和原位杂交方法对大鼠缺血60分钟再灌注肾组织ET?1及其受体亚型(ETA、ETB)的基因表达进行了研究。
- Kumark Saivithriy S. MRNA levels of Ca2+-independent form of protein kinase C in poslinchemic gerbil brain by northern blot analysis [J],J Biol Chem, 1997,252(61):7603. 卢布峰;鲁友明;黄诒森;蛋白激酶C对大鼠脑缺血海马突触体谷氨酸摄取的调控作用[J].;生物化学杂志;1994;10(3):371
- Methods such as Northern blotting, physiological and biochemical analysis were used to examine the genie expression of HSP70 and AC, the content of exohormone(IAA,ABA), and the change of legume abscission rate. The main results were shown as follows. 通过Northern分子杂交和生理生化分析测定等方法,检测处理后不同大豆品种植株花英离层区细胞HSP70和AC基因表达量、内源激素(ABA、IAA)含量以及花荚脱落率等各项指标的变化,研究这些指标之间的相互变化规律,其主要结果概述如下:
- Northern blot indicate that human BRI_3 mRNA is expressed principally in the brain, with the highest levels found at the cerebral cortex, medulla, amygdala, hippocampus, thalamus, caudate nucleus and spinal cord. BRI_2基因单碱基突变造成的通读和核苷酸重复,是基因水平上导致这两种痴呆症的主要原因。 该基因属于BRI基因家族的一员,目前得到分离鉴定的该家族成员包括BRI_1,BRI_2和BRI_3。
- Northern blot showed that A4-1 clone was specifically expressed in the antenna of H. armigera and the expressed level was higher in male antenna than that in female ones, which was similar to the expression of PBP-Harm gene from cotton bollworm. Northern杂交表明,克隆A4-1在棉铃虫触角中专一性表达,并且在雄蛾触角中的表达量明显比雌虫中高,这与棉铃虫性外激素结合蛋白基因的表达相似。
- Results of Northern blot showed that GOBP2 gene expressed specifically in the antenna of SPodoPtera exigua, and the expression level is consistent in the antenna of male and female moths. Northern杂交结果表明,GOBP2基因在甜菜夜蛾触角中特异性表达,并且在雌雄蛾触角中表达水平相当。
- We obtain 15 differentially expressed genes to human glioma thruogh four times hybridizations and scanning. Northern blot analysis confirmed 436F11 clone was over expression in human brian tissue and low expression in human glioma tissues. 通过四次基因芯片筛选 ,获得 15条与胶质瘤相关的新基因 ,经northernblot证实 436F11基因在人正常脑组织中高表达 ,而在人脑胶质瘤中低表达。