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- The egl gene was transferred to Saccharomyces cerevisiae on a multicopy plasmid or a CEN/ARS plasmid, and was expressed in recombinant S. cerevisiae H1m, H1p and H2p. 通过电转化使重组质粒转移至实验室酿酒酵母H158菌株中,分别得到了3株酵母转化子H1p、H2p和H1m。
- Finally, the linear pBLI plasmid vector coated with liposomere agent,was transferred into the bFF to validate pBLI. 最后,以线性化的乳腺特异表达载体pBLI为外源基因,采用脂质体介导法转染牛胎儿成纤维细胞。
- The researchers then used a plasmid vector to transiently increase levels of oct4 in hESC. 科学家们利用基因抑制技术（knockdown）来降低oct4基因的表达，这是一种对老鼠和人类中胚胎干细胞进行自我更新非常关键的基因。
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- We applied real-time image and fluorescence recovery after photobleaching (FRAP) to examine the nuclear pore permeability of 27 kDa EGFP in human lung cancer cells (ASTC-a-1) transfected with plasmid vector (pEGFP-C1). 本实验利用荧光漂白后恢复(FRAP,fluorescencerecoveryafterphotobleaching)法观测人肺腺癌肿瘤细胞(ASTC-a-1)中表达的27kDaEGFP在核质间的被动扩散,并以激光共焦显微镜进行实时成像。
- The recombinant Herpesviruses of Turkey transfer plasmid vector pT-C-gB-IL was cotransfected with Herpesviruses of Turkey at CEF by calcium phosphate-DNA coprecipitation method and the target fragment was inserted into TK domain of HVT. 确定转染时亲本毒的用量,用磷酸钙共沉淀法将HVT转移质粒载体pT-C-gB-IL与HVT在CEF细胞上进行同源重组,将目的基因导入HVT TK区。
- METHODS: Bcl-XL siRNA and negative siRNA plasmid vector were stably transfected into A549/DDP cells. Drug sensitivity of the cells to cisplatin(DDP) was analyzed with MTT and flow cytometry. 方法:将Bcl-XLsiRNA质粒载体转染至A549/DDP细胞,MTT、流式细胞仪检测细胞药物敏感性的改变;
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 应用脂质体Lipofectamine2000转染人结肠癌LoVo细胞,转染成功后以G418筛选4周,实时荧光定量RT-PCR(RealTimeRT-PCR)检测cerb-B1基因的mRNA表达,Western-blot检测EGFR蛋白的表达。
- The Situation of Plasmid Vector in Gene Therapy 以质粒为载体的基因治疗现状
- Plasmid Vector Suitable for Gene Therapy or Immunization 一种基因治疗和基因免疫表达载体的构建
- CONSTRUCTION OF SOIL METEGENOMIC LIBRARY WITH PLASMID VECTOR 以质粒载体构建土壤宏基因组文库的研究
- multicopy plasmid 多拷贝原粒
- Human lung cancer cells (ASTC a 1) were infected with EGFP plasmid vectors, and incubated in RPMI 1640 culture medium supplemented with 15% FCS and 800 mg/L G418. Strong fluorescence of five cell clones were observed after several generations. 以绿色荧光蛋白 (GFP)作为标记基因转入人类肺癌细胞系 (ASTC a 1) ;经 80 0mg/LG4 18筛选 ;获得5株高表达细胞系 .
- In this paper, the research in optimization design for DNA vaccine was reviewed, including choice of antigen gene and immune adjuvant, optimization of plasmid vectors and inoculation methods. 许多试图增强和调节质粒DNA疫苗的免疫原性和效率的方案都在研究之中。
- CONSTRUCTION OF NOS/HuIFNa CHIMERIC GENE AND ITS FUSION WITH Ti PLASMID VECTOR NOS/HuIFNa嵌合基因的构建及其与Ti质粒载体的拼接
- The Construction of Shuttle Plasmid Vector of Streptomyces and E. colt 链霉菌和大肠杆菌穿梭质粒载体的构建
- Construction of Plasmid Vector with Tat and the Study of Its Ability Transduct Fusion Protein into Cells 带Tat标记质粒载体的构建及其转导融合蛋白进入细胞的研究
- There are 3609 singlet genes and 745 multicopy genes. 使用STACK_PACK软件进行聚类分析,得到4354个基因聚类，包括3609个singlet基因和745个多拷贝基因；
- Construction of a low molecular weight plasmid vector for the capture of genes encoding exported proteins 捕获输出蛋白编码基因的小分子量质粒载体的构建与验证
- Construction of the shuttle plasmid vector for fusion protein gene and expression in Hepatocellular Carcinoma cells 携带融合基因穿梭质粒的构建及在人肝癌细胞中的表达
