The recombinant Herpesviruses of Turkey transfer plasmid vector pT-C-gB-IL was cotransfected with Herpesviruses of Turkey at CEF by calcium phosphate-DNA coprecipitation method and the target fragment was inserted into TK domain of HVT.

• 确定转染时亲本毒的用量,用磷酸钙共沉淀法将HVT转移质粒载体pT-C-gB-IL与HVT在CEF细胞上进行同源重组,将目的基因导入HVT TK区。