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- DNA yield rate of modified CTAB method was higher than modified SDS method. 改进的CTAB法DNA得率高于改进的SDS法；
- A modified CTAB method of genomic DNA extraction of Aquilaria sinensis(Lour. 在传统CTAB法的基础上加以改良,建立了一种适于白木香基因组DNA提取的方法。
- A modified CTAB was developed to isolate DNA of Chinese cabbage from each line and parents. 提取大白菜总基因组DNA以改良CTAB法为宜。
- The Genomic DNA samples of 15 taxa citrus were isolated by modified CTAB method and then AFLP analysis was conducted. 利用改进的CTAB法提取了15个柑橘分类群的基因组DNA,并进行了AFLP分析。
- Results shown that the PCR product of bacterial nucleic acid, which was purified with modified CTAB method, obtained stablethe most bands in DGGE. 结果表明,改良CTAB法抽提获得细菌核酸的PCR产物在DGGE凝胶上获得的条带最多,结果稳定。
- DNA extract by using modified CTAB methods was suitable for PCR-based detection of genetically modified organisms in foods and animals feed. PCR方法检测转基因食品时,应用改良CTAB法制备DNA模板。
- The results were as follows:1 .The genomic DNA of walnut was extracted by a modified CTAB method. The modified isolation buffer was: 100 mmol/L Tris-HCl (pH 8.0). 确定了适于AFLP分析的核桃基因组DNA提取方法为改良CTAB法;其提取液成份为:100mmol/L Tris-HCl;pH 8.;0;
- DNA bands showed that the modified CTAB and carbamide methods are better than the other four methods including CTAB, carbamide, high salt and low pH, SDS and Chen methods. DNA条带显示改良CTAB法和改良尿素法较好。
- By using high salt and low pH DNA method,the DNA extracted with high purity and low production,which of modified CTAB with the contrary results,high production and low purity. 用两种提取方法从叶片中得到的DNA产量和质量有所不同,高盐低pH法提取的DNA纯度高,但是产量较低; 而改良CTAB法则相反,产量高,纯度低。
- Pure total RNA and mRNA were successfully isolated from succulent halophyte,Salicornia bigelovii Torr. ,using a combining method of modified CTAB and commercial kit(Roche). 为提高缩叶肉质盐生植物海蓬子总RNA和mRNA的质量,将改良CTAB法与Tripure试剂盒方法相结合,从300 mg海蓬子发芽后20 d的幼苗中成功分离和纯化了高纯度的总RNA和mRNA。
- Comparison of different kits and different methods indicates that DNA with high purity and high yield can be extracted from vermicelli by the modified CTAB method. 本实验采用改良的简易CTAB法从深加工产品粉丝中获得高纯度和高浓度的DNA。
- Methods The extracts of high quality genomic DNA was extracted from the frozen leaves of Lycium bararum by modified CTAB method and its fingerprint was analyzed by RAPD. 方法采用改进的CTAB法提取枸杞冷藏幼叶的基因组DNA,利用RAPD技术对其进行指纹图谱分析。结果改进的CTAB法提取的10份枸杞材料基因组DNA均适于RAPD分析;
- Using the leaves of walnut Yangbi variety(Juglans sigillata) of different growing stage as the experimental materials, the genomic DNA extraction effect of two methods namely high salt and low pH and modified CTAB was compared. 以漾濞核桃不同生长期的叶片为材料,采用高盐低pH法和改良CTAB法作提取其基因组DNA的效果比较实验。
- The study of detection of 35S? NOS? Bt in 5 transgenic and 3 non-transgenic cotton seeds was carried out with DNA extraction by means of modified CTAB method and modified kit method respectively. 对5种转基因棉籽和3种非转基因棉籽,应用改良CTAB法和改良KIT法提取DNA模板,对35S、NOS、Bt等基因进行了检测研究,并确定了在检测Bt基因时的灵敏度。
- The CTAB-guanidinium method,modified CTAB method,and RNAplant Kit method were used to extract the total RNA from the plantlets of apple cultured in vitro,in which contain a lot of carbohydrates and phenolic compound. 利用CTAB_异硫氰酸胍法、改良CTAB法和RNAplant试剂盒法,从富含多糖和酚类物质的苹果组培苗继代苗中提取总RNA。
- The total RNA was extracted from riped "Red Bartllet" fruit by modified CTAB method. Two fragments of f3h gene which related to anthocyanin biosynthesis were cloned by RACE method. 论文第二部分以改良CTAB法从成熟红巴梨果皮中提取总RNA，并以此为模板，使用RACE方法成功分离出与花青素生成相关基因f3h基因cDNA的两个片段。
- The grain-specific endogenuous reference genes w ere amplified successfully when using DNAs extracted with modified CTAB method. 0.5% RRS and 0.5% Bt? 176 Maximaizer could be identified by PCR detecting c amv 35? 改良CTAB法制备的DNA用作PCR模板均可扩增出内参照基因 ,PCR扩增camv35S启动子可检测出含量为 0 5%25的RRS和Bt176Maximaizer,而PCR扩增nos终止子可检测出含 1%25RRS的食品样品。
- By comparing the result of between modified CTAB protocol and modified SDS protocol for isolating total RNA from Chinese jujube, it was found that modified SDS protocol was better than another on the quality of total RNA. 通过比较改良CTAB法和本试验设计的SDS法对枣果实总RNA的提取结果,发现本研究设计的SDS法提取总RNA的质量更高,效果更好。
- The modified CTAB and RNAplant Kit methods were a bit short in total RNA extraction because of a little DNA contamination. And the RNAplant Kit method showed advantages of fastness and convenience. 改良CTAB法和RNAplant试剂盒法提取的RNA均有少量DNA污染,但RNAplant试剂盒法比CTAB法更快捷,简便。
- An modified CTAB method, which can successfully remove phenolic compound, polysaccharide and other small molecule organic compound , was used to obtain total DNA from the leaf tissues of Acer miaotaiense . 研究结果表明：用改良的CTAB法提取庙台槭总DNA的效果良好，可有效地去除酚类化合物、多糖、小分子有机物等杂质的干扰，提取物可用于随后的多态性分析。
