The grain-specific endogenuous reference genes w ere amplified successfully when using DNAs extracted with modified CTAB method. 0.5% RRS and 0.5% Bt? 176 Maximaizer could be identified by PCR detecting c amv 35?

• 改良CTAB法制备的DNA用作PCR模板均可扩增出内参照基因 ,PCR扩增camv35S启动子可检测出含量为 0 5%25的RRS和Bt176Maximaizer,而PCR扩增nos终止子可检测出含 1%25RRS的食品样品。