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- metabolizing feeder layer cell 代谢饲养层细胞
- MEF, bEF, hEF feeder layer cells all can promote the efficiency of the isolation and clone human ES-like cells derived from PGCs. MEF、bEF、hEF三种饲养层细胞对人PGCs体外培养均有促进作用。
- The best pretreatment condition of STO feeder layer cells in transgenic mice STO转基因小鼠饲养层细胞的最佳预处理条件
- A new tissue engineering technique: culture and pretreatment conditions of feeder layer cells of embryonic stem cells Jun-Tao Han 组织工程新技术:胚胎干细胞滋养层细胞的培养及其预处理条件
- CONCLUSION: Mouse blastula can develop into embryonic stem cells on the fibroblast cell feeder layer, and can be passaged. 结论:小鼠囊胚在胚胎成纤维细胞饲养层上可发育成胚胎干细胞,并能进行传代。
- MAIN OUTCOME MEASURES: Preparation of fibroblast feeder layer;growth of embryonic stem cells;results of alkaline phosphatase staining;cell differentiation tendency. 主要观察指标:成纤维细胞饲养层的制备情况,胚胎干细胞的生长状态、碱性磷酸酶染色结果及分化趋势。
- effect of the density of feeder layer cells on the growth of embryonic stem cells: 10 mg/L MMC group could achieve the best pretreatment effect, and STO cells of this group were selected as feeder layer cells to culture D3-embryonic stem cells. 饲养层细胞密度对胚胎干细胞培养的影响:本实验中10mg/L丝裂霉素C处理组预处理效果最佳,取该处理组作为饲养层培养D3系小鼠胚胎干细胞。
- Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast(MEF) cells for isolation and culturing of embryonic stem(ES) cells. 目的:建立ICR小鼠胚胎成纤维细胞饲养层培养体系,用于胚胎干细胞建系及研究。
- ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering. 在复苏后早期阶段ES细胞培养中 ,不宜单独应用BRL条件培养基 ,须用MEF饲养层或合并应用BRL条件培养基和MEF饲养层
- Zhou JX, Liu D, Zheng KL, Deng CH, Wen RQ, Tang LX, Ma CJ.Culture conditions of mouse embryonic fibroblasts and preparation of feeder layer. 关键词:胚胎成纤维细胞;
- CONCLUSION: Endometrial fibroblast is considered to be feeder layer of PGC.After passage, PGC remains undifferentiation and pluripotent of embryonic stem cells. 结论:人子宫内膜成纤维细胞可以作为人原始生殖细胞的饲养层细胞,传代后的原始生殖细胞仍保持胚胎干细胞的未分化状态和发育全能性。
- The superficial layer cell of basic petal becomes matic activity and formes the primordium nectary.The pre-nectar may be provided by phloem that is round the nectariferous tissue. 原蜜汁由蜜腺周围的维管束提供,经产蜜组织加工后,由分泌表皮外薄的角质层泌出。
- Under appropriate conditions, the adherent cells from HUCB could form the feeder layer to support long-term hematopoiesis and provide the supporting stromal cells for the ex vivo expansion of HSPCs. 在适当的条件下;可以形成饲养层细胞;为造血干/祖细胞体外扩增提供支持基质细胞.
- OBR positive cells were also present in choroid plexus, brain ependymal layer cell and vascular endothelial cells. OBR阳性细胞表达于脉络丛、脑室管膜内皮细胞和脑血管内皮细胞;
- When cultured on degenerated feeder layers or in suspension, EG cells formed embryoid bodies (EBs) in vitro. 当EG细胞脱离饲养层悬浮培养,或在衰老的饲养层上延迟培养时,发现EG细胞或单个存在,或聚集成团,形成类似于早期胚胎的囊状胚体结构.
- Methods Neomycin gene was transfected into human embryonic fibroblasts (HEF) by liposome mediated method. Using this transfected HEF as feeder layer, ES cells were transfected with AANAT gene. 方法 将外源性neo基因转染人胚胎成纤维细胞 ,作为饲养层细胞用于ES细胞转基因后筛选及增殖过程的培养。
- METHODS: PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture. 方法:从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞,将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
- The nectary originates from the several outer layer cells of base of carpel primordia. 蜜腺起源于心皮原基基部外侧的几层细胞。
- The little boy is a noisy feeder. 这个小男孩吃东西时发出响声。
- Conclusions After phenol chemical peeling,the melansomes in cuticular layer cells decrease... 结论雀斑化学剥脱术后,表皮层细胞黑色素颗粒数量明显减少,雀斑消失;