effect of the density of feeder layer cells on the growth of embryonic stem cells: 10 mg/L MMC group could achieve the best pretreatment effect, and STO cells of this group were selected as feeder layer cells to culture D3-embryonic stem cells.

• 饲养层细胞密度对胚胎干细胞培养的影响:本实验中10mg/L丝裂霉素C处理组预处理效果最佳,取该处理组作为饲养层培养D3系小鼠胚胎干细胞。