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- Cecropin-XJ expressed in Pichia pastoris was purified through ion exchange chromatobraphy(IEC),hydrophobic interaction chromatography(HIC) and size exclusion chromatography(SEC). 通过离子交换层析、疏水作用层析和分子排阻层析等三步层析的方法,分离纯化了毕赤酵母工程菌所表达的新疆家蚕抗菌肽。
- Based on the property of bacterial endotoxin,the effects of affinity chromatography,ion exchange chromatography and hydrophobic interaction chromatography on removal of endotoxin in the pu- rification of recombinant12 5Ser- IL - 2 were compared. 针对细菌内毒素的化学性质 ,比较了用亲和层析法、离子交换层析法和疏水相互作用层析法等 3种层析法去除基因重组 1 2 5Ser- IL- 2产品中细菌内毒素的效果。
- Methods: Wash inclusion body effectivty, use Hydrophobic Interaction Chromatography to carry on the purification. 方法：对包涵体进行有效洗涤，采用疏水层析进行纯化。
- Hydrophilic interaction chromatography (HILIC) is popular in the analysis of hydrophilic solutes in recent years. 摘要亲水作用色谱(HILIC)是近年来色谱领域研究的热点之一。
- The retention behaviors of aromatic alcohol homologues in hydrophobic interaction chromatography (HIC) were investigated firstly. 首次研究了芳香醇同系物在疏水色谱(HIC)中的保留行为。
- Then the refolding of protein in the ion-exchange chromatography (IEC), hydrophobic interaction chromatography (HIC) and size-exclusion chromatography (SEC) was studied. 所以本文将首先从描述液-固界面的模型入手，研究溶质在液-固界面的吸附行为受到的影响，在此基础上对变性蛋白在离子交换色谱(IEC)，疏水色谱(HIC)及排阻色谱(SEC)中的复性进行研究。
- The silicagel column has showed an irreversible adsorption equilibrium for epirubicin and its analogues with slightly acidic or neutral mobile phases in hydrophilic interaction chromatography (HILIC). 亲水作用色谱分离表阿霉素时,在中性及弱酸性条件下,样品在硅胶柱上呈现不可逆吸附平衡过程。
- Hydrophobic interaction chromatography (HIC) is widely used in protein separation and purification.Butyl-agarose，phenyl-agarose and octyl-agarose are the most important HIC media. 疏水层析是广泛应用于生物大分子分离纯化的技术，丁基琼脂糖（butyl-agarose）层析介质、苯基琼脂糖(Phenyl-agarose)层析介质、辛基琼脂糖（octyl -agarose）层析介质是常用的疏水分离介质。
- hydrophobic interaction chromatography 疏水作用色谱法
- thiophilic interaction chromatography 亲硫作用层析
- hydrophilic interaction chromatography 亲水作用色谱
- hydrophilie interaction chromatography 亲水作用层析
- hydrojphilie interaction chromatography 亲水作用色谱
- hydrophilic interaction chromatography(HILIC) 亲水作用色谱
- hydrophobic interaction chromatography(HIC) 疏水作用色谱
- metal chelate interaction chromatography 金属螯合作用层析
- metal interaction chromatography 金属作用层析
- Mother and baby interact in a very complex way. 母亲与婴儿以非常复杂的方式相互影响。
- Keywords ribonuclease;protein refolding;hydrophobic interaction chromatography;ion exchange chromatography; 关键词核糖核酸酶;蛋白折叠;疏水作用色谱;离子交换色谱;
- Keywords salt lake brine system containing lithium;osmotic coefficient;isopiestic method;ion interaction model; 含锂盐湖卤水体系;渗透系数;等压法;离子相互作用模型;
