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- in vivo transient transfection 体内瞬时转染系统
- Adl2 ElB 55kDa binds hDaxx in vivo or in vitro. 共免疫沉淀反应和Western blot结果证实Ad12 E1B 55KD能在细胞内外与hDaxx直接结合。
- The expression product shows activity of antitumor in vivo. 表达产物在体内具有一定的抗肿瘤活性。
- Result Nicardipine can induce rat mesangial cell apoptosis in vivo. 结论尼卡地平在体内有诱导大鼠系膜细胞凋亡的作用。
- Transient transfection shows that GCRG213 is located in cytoplasm and nuclei of COS-7 cells. GCRG213蛋白在胞核和胞质中均有表达。
- Methods Using the in vivo micropuncture technique in SD r ats. 方法利用活体微穿刺技术。
- CONCLUSION Survivin T34A might inhibit A549 tumors growing in vivo. 结论 Survivin T34A在体内可明显抑制A549肺癌生长。
- The transient transfection of Stat 3 cDNA into COS7 cells increased Stat3 protein level and the expression of the reporter gene. Stat3cDNA和报道基因在COS7细胞中的共转染实验表明 ,外源的Stat3蛋白具有DNA结合活性 ,并可增强报道基因的表达。
- At last, tumorigenesis assay was undertaken in nude mice in vivo. 进行黑素瘤细胞体外克 隆形成实验观察,计算各组细胞的克隆形成率;
- Transient transfection of Stat3 cDNA into COS7 cells increased Stat3 protein level and also the reporter gene expression. Stat3 cDNA和报告基因在COS7细胞中的共转染实验表明，外源的Stat3蛋白具有DNA结合活性，并可增强报告基因的表达。
- The embryos from the in vivo fertilization develop best. 体内受精胚胎的体外发育最佳。
- The mount of vectors and polybrene, the hatching time for them were optimized through transient transfection of pG-CMV-DRE(2). 通过瞬时转染，在转染了载体pG-CMV-DRE(2)和pG-SV-DRE(2)的细胞体内观察到有报告基因绿色荧光蛋白表达。
- And, these monkeys were injected in vivo dose must SIV virus. 然后，这些猴子体内均被注入一定剂量的SIV病毒。
- Conclusion The VH containing extra-sequence may exist in vivo. 结论提示这段异常序列在体内可能存在于该VH之中。
- Recombinant retrovirus was produced by transient transfection of the Phoenix-ecotropic packaging cell line with pMX-3FLAG-Id3-EGFP vector using FuGene-6 transfection reagent. 通过脂质体转染技术将重组逆转录病毒载体(pMX-3FLAG-Id3-EGFP)转染Phoenix-ecotropic包装细胞系;
- The study of gene transfection into rabbit's tongue muscle in vivo and optimization. 分子外科缝线术的建立及其优化方案的研究。
- Transgenic chicken could be obtained by transfection the PGCs in vivo or in vitro and screening the offsprings. 对鸡PGCs进行体外或者体内转染外源基因操作，再通过后代的筛选，就能获得转基因的后代。
- Incerase in serum NSE provides further in vivo evidence of transient brain injury after epileptic seizure. 癫痫发作组NSE水平明显高于正常对照组 ,提示癫痫发作后有一定程度的脑神经元损害。
- After transient transfection into K562 cells, real-time quantitation PCR was used to detect the expression alteration of PCGF4/Bmi-1, and growth curve was utilized to observe the alteration of growth rate of cells. 转染K562细胞,瞬时干扰PCGF4/Bmi-1基因的表达,实时定量PCR检测干扰前后基因表达变化。 利用生长曲线测定干扰载体转染前后细胞生长速度变化。
- After transient transfection of COS 7 cells with mixture of equal amount of the two plasmids, the antigens JEV E and HBV preS1 were both detected in the culture supernatant by ELISA with corresponding monoclonal antibodies. 等量混合这两种质粒，转染COS?7细胞进行瞬间表达，应用单克隆抗本ELISA法检测特异性抗原。
