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- Conclusion The hybridoma cell line may be applicable to the diagnosis of HBsAg. 结论筛选出了一株适合临床应用的单抗杂交瘤细胞株。
- Results A hybridoma cell line secreting McAb steadily with high titer was obtained, which was named SU-2000. 结果 得到1株稳定分泌抗体、效价高的杂交瘤细胞株,命名为SU-2000。
- The monoclone hybridoma cell line obtained was named SRBC-C, which secreted anti-human B grouping red cell antibody and was used for ABO blood typing. 该株能分泌抗B抗体,与B型红细胞发生凝集,对鉴定ABO血型有一定价值。
- The effects of glucose, L-glutamine, ammonia and lactic acid on the growth of mouse-mouse hybridoma cell line G_5F_4 were studied. 研究了葡萄糖、谷氨酰胺、氨和乳酸对鼠鼠杂交瘤细胞G_5F_4生长的影响。
- The hybridoma cell line with the collection number of CGMCCNO.1908 is used for a secrete anti-URG11 monoclonal antibody hybridoma cell line. 所述的保藏号为CGMCCNO.;1908的杂交瘤细胞株用于分泌抗URG11单抗的杂交瘤细胞株。
- The Balb/c mouse was immunized with antigen of purified avian encephalomyelitis virus strain Van Roekel which was adapt for cell culturing. A hybridoma cell line, which can secret monoclonal antibodies against avian encephalomyelitis(AEV), was obtained. 利用提纯的禽脑脊髓炎病毒 (AEV)VR株CEF适应毒制备抗原 ,免疫BALB/c小鼠 ,应用杂交瘤技术以间接ELISA筛选获得 1株分泌抗AEV(VR)单克隆抗体的杂交瘤细胞。
- The hybridoma cell lines could produce the McAbs which was specific to BDV P24 and P40 antigens with the appropriate titers. 杂交瘤细胞系H1和H2可以产生一定效价的单克隆抗体,并对BDV的P24和P40抗原具有特异性。
- RESULTS: Four hybridoma cell lines targeting a suitable peptide from pf HRP-II were obtaind by using spleen embedment method and hybridoma technology. 结果: 应用脾脏埋植法和杂交瘤技术, 获得4 株针对恶性疟原虫HRP-II单一表位的杂交瘤细胞。
- Conclusion Plasmodial aldolase has been successfully expressed and purified, and the established hybridoma cell lines can secrete McAbs specific to the aldolase of P. falciparum. 结论 本实验构建并表达了重组疟原虫糖酵解醛缩酶,并获得特异性的单克隆抗体。
- The average numbers of chromosomes of the two hybridoma cell lines were 98 and 101 which were consistent with the total chromosome numbers of spleen cell and myeloma cell. 染色体计数分析结果显示,两株杂交瘤细胞的平均染色体数分别为98和101,在脾细胞与 SP2/0 细胞染色体数之和的范围之内;
- The McAbs anti TAP were obtained by screening with indirect ELISA and 4 times clone, and were to be preliminary characterized. Results The rate of fusion was 85.03%. Ten hybridoma cell lines were developed which could secret McAb stably. 结果细胞融合率85.;03%25;经4次克隆化后获得10株能稳定分泌TAPMcAb的杂交瘤细胞株;经免疫球蛋白亚类鉴定;除1株为IgG1外;其余9株均为IgM类;
- The cTnI was used to immunize Balb/c mice by route method. The spenocytes were fused with SP2/0 myeloma cell lines. Six hybridoma cell lines,secreting stably McAbs to cTnI antigen have been obtained and screened. 方法 :应用cTnI免疫Balb/c小鼠 ,取脾细胞与SP2 /0骨髓瘤细胞进行细胞融合筛选 ,获得分泌高效价抗cTnI抗体细胞株 6株。
- In the research, five hybridoma cell lines (1A4,2C7, 3G9 3D5 and 2G3 ) secreting monoclonal antibodies(McAbs) against Aeromonas sobria CR79-1-1 strain were established by the fusion technique. 经单抗亚类试剂盒测定,3株单克隆抗体2G3、1A4和3G9蛋白均为IgG,亚类分别为:IgG2_b,IgG1,IgG1。
- The BALB/c mice were immunized with the whole antigen GSH-S-DNP-BSA,and hybridoma cell lines 4A4, 6A6, 1C8 and 4G3 which secreted monoclonal antibodies specific to GSH were established by lymphocyte hybridoma technique. 用全抗原免疫BALB/c小鼠,通过淋巴细胞杂交瘤技术,制备出抗GSH的单克隆抗体杂交瘤细胞系4A4、6A6、1C8和4G3等。
- Keywords Tetanic toxoid;Hybridoma cell line;Monoclonal natibodies; 破伤风类毒素;杂交瘤;单克隆抗体;
- Five hybridoma cell lines producing monoclonal antibodies (McAb) against aflatoxin B1 (AFB1) were established after fusion of mouse myecloma cells (Sp-2/0-Ag-14) with spleen cells isolated from male BALB/c mice immunized with AFB1-BSA conjugate. 用杂交瘤技术制备了5株产生抗黄曲霉毒素B_1单克隆抗体的杂交瘤细胞株。 对其中之一AFB_1-2H8进行了较系统的研究。
- The preliminary determination of anti-TPO monoclonal antibody hybridoma cell line 抗TPO单克隆抗体杂交瘤细胞系的建立
- Keywords Ovalbumin;Monoclone antibody;Hybridoma cell lines; 卵清蛋白;单克隆抗体;杂交瘤细胞株;
- Construction of Apo B_(100) Hybridoma Cell Line and Study on Immunological Characterization of McAb 载脂蛋白B_(100)杂交瘤细胞株的建立及单抗免疫学特性的研究
- Maintain the cell line equipments. 负责尚能电池线设备维护。