The McAbs anti TAP were obtained by screening with indirect ELISA and 4 times clone, and were to be preliminary characterized. Results The rate of fusion was 85.03%. Ten hybridoma cell lines were developed which could secret McAb stably.

• 结果细胞融合率85.；03%25；经4次克隆化后获得10株能稳定分泌TAPMcAb的杂交瘤细胞株；经免疫球蛋白亚类鉴定；除1株为IgG1外；其余9株均为IgM类;