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- Purification: Density Gradient Centrifugation (Sucrose &CsCl). 分离:密度梯度离心法(蔗糖&氯化铯).
- Methods LDL was separated by density gradient centrifugation. 方法密度梯度离心法分离人低密度脂蛋白;
- Five zones were separated by improved discontinuous sucrose gradient centrifugation. 用改进的不连续蔗糖密度梯度离心法分离到五条带。
- Conclusion:High purity of MSC can be obtained by density gradient centrifugation. 结论:密度梯度离心法分离获得的人胚胎骨髓间充质干细胞纯度高。
- Methods Peripheral blood monocytes were obtained from PVS patients and normal control group by density gradient centrifugation. 方法 应用密度梯度离心法将实验组即PVS病人及正常对照组的外周血单核细胞分离出来 ,然后应用酶联免疫法检测外周血单核细胞受IFN r或LPS刺激后 ,其所表达的HLA DR的变化水平。
- The karyotes were separated by density gradient centrifugation, and the stroma cells of the bone marrow were gained adherent separation method. 选择雄性6周龄SD大鼠4只,抽取大鼠股骨骨髓,使用密度梯度离心分离有核细胞,贴壁分离法获取骨髓基质细胞,以2×104/cm2的密度接种进行细胞传代与分组实验。
- We generally purify plasmids from bacterial proteins, chromosomal DNA and RNA using cesium chloride gradient centrifugation. 我们通常用氯化铯分级离心分离把质粒从细菌蛋白、染色体DNA和RNA中纯化出来。
- Methods Type A spermatogonial stem cells were isolated from 9 day-old SD rat testes by discontinuous Percoll gradient centrifugation. 目的摸索完善A型精原干细胞分离、纯化的方法与技术。
- In this study, the purified and concentrated oocysts were isolated from the faecal samples by density gradient centrifugation. 本研究以不同密度的蔗糖溶液为介质,按比例配制两种密度的分离液,对采自广东省的奶牛粪便进行分离纯化,得到了较纯净的牛源环孢子虫卵囊;
- Methods Samples of peripheral blood in 64 women of 8-40 gestational weeks were collected to enrich the fetal nucleated red blood cells by density gradient centrifugation. 方法对64例孕8-40周孕妇外周血进行密度梯度离心法,富集胎儿细胞,并经显微镜下观察计数各种细胞所占百分比。
- Methods Using different techniques of collagenase digestion and discontinued density gradient centrifugation, the adult swine and rat islets were prepared. 方法采用不同的胶原酶消化法及不连续密度梯度纯化法。
- Marrow mesenchymal stem cells can obtain both with adherence separation and density gradient centrifugation by suitable culturing condition. Import FBS and F12-DMEM nutritive medium are better choice. 采用全骨髓贴壁分离法和密度梯度离心法,只要选择合适的培养条件均可获得骨髓间充质干细胞,但选用进口胎牛血清、F12-DMEM培养基效果较好。
- The fetal liver stem cells were isolated by collagenase digestion, gravity sedimentation and density gradient centrifugation, identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition. 采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞,通过免疫细胞化学方法对其进行初步鉴定,以及应用流式细胞仪等对其生长状况进行评估。
- Methods Mononuclear cells were isolated from human peripheral blood cells by density gradient centrifugation and cultured in M199 medium with fetal blood serum(FBS),vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF). 方法用密度梯度离心法从人外周血中分离单个核细胞。 在加有胎牛血清(fetal blood serum,FBS)、血管内皮生长因子(vascular end othelial growthfactor,VEGF)和碱性成纤维生长因子(basic fibroblast growthfactor,bFGF)的M199培养液中培养。
- Index detection: Growth condition and morphologic feature were observed in different culture conditions of adherence separation and density gradient centrifugation under inverted microscope. 指标检测:倒置显微镜下,每天观察贴壁分离、密度梯度离心不同培养条件下细胞的生长情况和活体形态特征。
- Having isolated peripheral blood mononuclear cells(PBMCs) from peripheral blood of normal person by Ficoll-Hypaque density gradient centrifugation,Total RNA were extracted according to Trizol kit(GIBCO/BRL). Identified by improved gel electrophoresis. 以Ficoll-Hypaque一步密度梯度离心法从健康人外周血中分离单个核细胞,按GIBCO/BRL公司的Trizol试剂说明书提取总RNA,用改进的凝胶电泳判断其是否降解。
- The Yucaipa Strain of PMV-2 virus, which is the standard strain of the virus, and other three isolated strains in China we re propagated in the allantoic cavity of SPF chicken eggs, and purified by disco ntinuous sucrose density gradient centrifugation. 将禽副粘病毒 2型的标准毒株Yucaipa病毒和国内分离株F4、F6和F8株分别接种SPF鸡胚 ,收获鸡胚尿囊液 ; 经差速离心和蔗糖密度梯度离心后 ,分别制备电镜样品和免疫电镜样品 ;
- METHODS: Bone marrow blood was sterilely collected.Human BMSCs were cultured in monoclone by density gradient centrifugation and adherence screening.Cells of 90% confluency were digested by trypsin. 方法:无菌条件下抽取骨髓血,联合应用密度梯度离心法及贴壁筛选法,以单克隆形式分离培养人骨髓间充质干细胞,当细胞汇合至90%25时胰蛋白酶消化传代。
- By differential centrifugation and sucrose density gradient centrifugation technology,a spherical virus and phage are isolated.It is important that this is the first report for Lentinus edodes phage. 利用差速离心和蔗糖梯度离心技术,从香菇子实体中分离出了一种球状病毒和一种噬菌体,其中噬菌体为国际首次在香菇中发现。