Methods Peripheral blood monocytes were obtained from PVS patients and normal control group by density gradient centrifugation.

 
  • 方法 应用密度梯度离心法将实验组即PVS病人及正常对照组的外周血单核细胞分离出来 ,然后应用酶联免疫法检测外周血单核细胞受IFN r或LPS刺激后 ,其所表达的HLA DR的变化水平。
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