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- Keywords xylose fermentation;genic recombinant strain;Saccharomyces cerevisiae;Zymomonas mobilis;Escherichia coli; 木糖发酵;基因工程菌;酿酒酵母;运动发酵单胞菌;大肠杆菌;
- genic recombinant strain 基因工程菌
- When administered orally to mice, the recombinant strain elicited a serum antibody response to CTB. 用重组菌株口服免疫接种小鼠,能够激发抗 CTB抗体的产生。
- Methods Gene hpaA amplified from Hp genome was cloned to pET- 28a( + ) vector, then the recombinant strain (pPHA/BL21) was induced to express rHpaA by IPTG. 方法采用PCR技术从幽门螺杆菌基因组中扩增hpaA基因,克隆至表达载体pET-28a(+)中,IPTG诱导表达重组蛋白HpaA(rHpaA),鉴定rHpaA蛋白的表达形式。
- Under 5% dissolved oxygen, the cell dry weight (CDW) of the recombinant strain and wild strain were 45.1 g/L and 54.1 g/L respectively, the concentration of Glu were 9.56 g/L and 1.27 g/L, the concentration of Gln were 5.51 g/L and 2.27 g/L. 对得到的重组菌和野生菌进行了发酵实验 .;在溶氧只有 5%25的条件下;重组菌比野生菌细胞干重提高了 1
- The positive recombinant strains(Mut+) were obtained by MM and MD plate and PCR analysis. 获得的菌体经超声破壁、硫酸铵沉淀得粗酶,再利用金属Ni螯和型树脂经一步分离纯化得到可转化头孢菌素C制备GL-7-ACA的DAAO。
- PUC18 transferring and ERIC-PCR showed that the recombination strain SR-15 could grow in the plant stably. SR-15菌株通过质粒PUC-18转化和ERIC-PCR再分离实验验证,结果显示重组菌株在植株体内稳定定位,具有稳定的内生特性。
- This strain of wheat can grow during a cold spring. 这种小麦可以在寒冷的春天生长。
- Keywords recombinant strain;glutathione;shake flask;fermenter; 重组酵母;谷胱甘肽;摇瓶;发酵罐;
- This review provides the current status of ethanol fermentation from dilute-acid hydrolysate of lignocellulose including main yeasts,bacteria,as well as construction of recombinant strains. 文章报道了国内外近年来利用木质纤维素稀酸水解液制取燃料乙醇的菌种的研究现状,主要包括木质纤维素稀酸水解液乙醇发酵的酵母菌、细菌及基因重组菌的菌种构建等。
- He finally break down under the four-hour's strain. 在四小时的压力下他终于支持不了。
- Paying all the bills is a strain on my resources. 支付所有这些费用是我经济上的一大负担。
- Her speech continued in the same dismal strain. 她以悲伤的语调接著往下说。
- Could you stand the strain? Oh, that's nothing. 这样累你受得了吗?这没什么。
- He broke under the strain of heavy work. 他在繁重工作的压力之下累坏了。
- There's a strain of madness in the family. 那一家人都有点疯疯颠颠的。
- He was breaking up under the strain. 他劳累过度,身体逐渐衰弱。
- The official was warned not to strain his power. 那个官员受到警告,不可滥用职权。
- He pitched his story in a sentimental strain. 他把故事写得带有伤感情调。
- Strain began to tell on his health. 过度劳累开始影响他的健康。