Methods Gene hpaA amplified from Hp genome was cloned to pET- 28a( + ) vector, then the recombinant strain (pPHA/BL21) was induced to express rHpaA by IPTG.

• 方法采用PCR技术从幽门螺杆菌基因组中扩增hpaA基因,克隆至表达载体pET-28a(+)中,IPTG诱导表达重组蛋白HpaA(rHpaA),鉴定rHpaA蛋白的表达形式。