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- The recovered bands of PrP, GFP and pCDNA3.1(-) were ligated with T4 ligase to produce a fusion expression plasmid, pCDNA3.1(-)-mPG, which was then used to transfect the Neuro-2a cell under the selection pressure of G418 antibiotics. 将这三个片段共连接;从而获得融合mPrP/GFP基因的真核表达质粒pCDNA3.;1(-)-mPG。 由于在PrP C-端融合了绿色荧光蛋白(GFP);使该系统具备了早期筛选的标签。
- Bovine Enterokinase Catalytic Subunit cDNA Cloning and Construction of Fusion Expression Plasmid 牛肠激酶催化亚基cDNA的克隆及融合表达载体的构建
- Construction of a fusional expression plasmid in human monocyte chemoattractant protein-1 人单核细胞趋化蛋白-1融合表达质粒的构建
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- Constructed the expression plasmid pTrc-rCR and checked it. 构建表达质粒pTrc-rCR,并酶切检验;
- Then, a constructed prokaryotic expression plasmid of pGEX-2T-E7 presented by Dr. Werner, was transformed into E. coli BL21(DE3 stain) and induced by IPTG to express GST-E7 fusion protein. 为了研究HPV16E7疫苗的需要，我们利用Werner博士惠赠的pGEX-2T-E7原核表达质粒转化入大肠扪：菌BL21(DE3)中，用IPTG诱导后，裂解细胞，用SDS-PAGE鉴定诱导效果，发现在43kDa处有融合蛋白的表达。
- Objective To construct HBVx-GFP eukaryotic expression plasmid and establish stable transfected HepG2 cell line expressing HBVx-GFP fusion protein for exploring the biological function of HBVx and its roles in carcinogenesis of hepatocellular carcinoma. 目的构建绿色荧光蛋白(GFP)与人乙型肝炎病毒(HBV)X基因的重组表达载体,建立稳定表达HBVX蛋白(HBx)与GFP融合蛋白的HepG2细胞系,以进一步研究HBx的生物学功能及其在肝癌发生中的作用。
- The prokaryotic expression plasmid pGEX-IL-24 was constructed,GST-IL-24 molecular mass was approximately 50 000.Conclusion GST-IL-24 fusion protein was expressed in E. coli BL21(DE3) which was transformed from the recombinant vector of IL-24. 结论IL-24的重组载体在大肠杆菌BL21(DE3)可以表达GST-IL-24融合蛋白。
- Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid. 目的克隆人血管紧张素转换酶2基因(ACE2),并构建其真核表达载体。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- Results The bicistronic eukaryotic expression plasmid was corrected and can co - express human BMP -2 and VEGF165 mRNA in vitro. 该重组质粒能在体外同时表达BMP2及VEGF165 mRNA。
- The co expression plasmid encoding FasL and VEGF165 named pCI FasL IRES VEGF165 (simplified as pCI FIV) was successfully constructed. 再以此为基础构建得到编码FasL和VEGF165的共表达质粒 pCI FasL IRES VEGF165 (简称pCI FIV )。
- Expression and purification of recombinant RTAsThe expression plasmid Pkk223.3-RTA was introduced into E. coli JM 109 by CaCl2-mediated method. 将构建好的重组质粒pKK223.;3-RTA和pKK223
- The experiment results covered several following points:(1) adw was cloned into nuclear plasmid PB, PBG and chloroplast expression plasmid PLCTB . 将乙肝表面抗原基因(adw型)与细胞核载体PB、PBG以及叶绿体表达载体PLCTB进行定向克隆，经PCR和测序鉴定都获得了重组子，分别命名为PB-adw、PBG-adw、PLCTB-adw。
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
- Methods: 1.The KK gene was directional cloned into the pAAV-MCS. Thisrecombinant pAAV expression plasmid was called pAAV-KK. 方法:1.;将KK 基因定向克隆入腺相关病毒载体质粒pAAV-MCS 中构建成pAAV-KK。
- SUMO protease can cut SUMO fusion protein expressed by fusion expression system without any amino acid residues left on target protein thus become a hot topic in this field. SUMO蛋白酶对SUMO融合表达系统表达的重组蛋白进行切割时没有多余氨基酸残留，因此成为蛋白切割工具的热点。
- To construct mammal expression plasmid pcDNA 3.1 ( + )/GDF-5 and check the expression of it in bone marrow mesenchyal stem cells of mice. 目的:通过基因重组技术体外构建真核表达质粒pcDNA3.;1(+)/GDF-5;并检测其在小鼠骨髓基质干细胞中的表达。
- Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根据GenBank S.;suis2epf基因序列设计引物;克隆ZYH24株epf基因片段并进行序列分析;
- Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物，并构建了pGEX-4T-1-BLY原核表达载体。 经BamHI和EcoRI酶切及质粒PCR鉴定，证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上，为进一步研究其诱导表达条件及生物学功能奠定了基础。
