The recovered bands of PrP, GFP and pCDNA3.1(-) were ligated with T4 ligase to produce a fusion expression plasmid, pCDNA3.1(-)-mPG, which was then used to transfect the Neuro-2a cell under the selection pressure of G418 antibiotics.

• 将这三个片段共连接；从而获得融合mPrP/GFP基因的真核表达质粒pCDNA3.；1(-)-mPG。 由于在PrP C-端融合了绿色荧光蛋白(GFP)；使该系统具备了早期筛选的标签。