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- The L ScFv genes were inserted into the eukaryotic fusion protein expression vector pEGFP N3 and transfected into COS 7 cells to express respectively. 将所构建的单链抗体基因克隆入绿色荧光蛋白 (GFP)融合表达载体pEGFP N3,并转染COS 7细胞进行表达。
- SUMO protease can cut SUMO fusion protein expressed by fusion expression system without any amino acid residues left on target protein thus become a hot topic in this field. SUMO蛋白酶对SUMO融合表达系统表达的重组蛋白进行切割时没有多余氨基酸残留,因此成为蛋白切割工具的热点。
- A human fusion protein expressed by E2A/HLF mRNA was found by the method of DD-PCR. RT-PCR confirmed that the level of E2A/HLF mRNA was higher in the part of stroke than that of control in brain tissue of stroke patients. 经DD-PCR筛选到17个差异片段,其中1个可能为出血性中风相关基因,即人类E2A/HLF融合蛋白mRNA,此基因经RT-PCR证实在出血性中风病人脑组织中高于正常组织。
- KIR2DL1 IgG Fc fusion protein expressed in COS 7 cells successfully and the protein can mimic the natural KIR2DL1 protein and is used as a potential tool in the recognition of its ligands. KIR2DL1 Ig融合蛋白表达载体成功构建并在COS 7细胞中获得功能性表达 ,为KIR2DL1的功能及其配体MHC的研究奠定了基础
- The result of RT-PCR and immunocytochemistry and western blotting studies revealed that less c-myc mRNA and EWS-Flil fusion protein expressed as time going on in the early stage of apoptosis induced by As2O3 in RD-ES cell. c-myc基因mRNA表达水平和EWS-FLi1融合蛋白表达量随As2O3作用时间延长逐渐降低。
- The fusion gene was cloned into pcDNA3.1(+) and the recombinant plasmid pcDNA Ag85B MPT64 was transfected into COS 7 cells. The fusion protein expressed in COS 7 cells was determined by RT PCR, ELISA and dot blotting. 采用脂质体转染法将pcDNA/Ag85B MPT6 4 (pcDNA/AM )转染COS 7细胞 ;用RT PCR、ELISA和斑点印迹法检测其表达 .
- Keywords Nicotinamide N-methyltransferase Antigen Fusion protein expression; 尼克酰胺N-甲基转移酶;抗原;融合蛋白表达;
- Keywords tyrosine hydroxylase;catalytic domain;wild type enzyme;fusion protein expression;specific activity of enzyme; 关键词酪氨酸羟化酶;催化结构域;野生型酶;融合蛋白表达;酶活性;
- interleukin-2 (IL-2)/interferon-γ(IFN-γ) fusion protein expression plasmid (pFP) 白细胞介素-2(IL-2)/γ-干扰素(IFN-γ)融合蛋白表达质粒(pFP)
- Objective To construct the GST fusion protein expression plasmid of cardiotrophin-1(CT-1) and tetanus toxin C frag-(ment(TTC).) 目的构建心肌营养素-1(CT-1)和破伤风外毒素C片段(TTC)重组融合蛋白表达质粒。
- Purified fusion protein was obtained. 得到纯化的融合蛋白。
- fusion protein expression 融合表达
- The GRA7 gene may be expressed as a GST fusion protein in E. coli. GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达。
- The expression was induced with IPTG and the fusion protein was confirmed to be 40.9 kDa by SDS-PAGE analysis. 重组菌经IPTG诱导培养后,SDS-PAGE电泳可见分子量为40.;9 kDa的重组蛋白;
- The protein expression of Bax was increased after DATS treatment. 免疫组化显示;经DATS处理后;Bax的表达增强(P<0.;05);Bcl-2表达未见明显改变。
- Contents include cell culture、cell fusion、Cytochemistry、apoptosis detection、protein expression and function determination,etc. 课程涉及的主要实验内容包括细胞培养、细胞融合、细胞化学、细胞凋亡检测和蛋白质的表达与功能检测等。
- The s-lap/GFP fusion protein can express in Hela cells and locate in entire cells, but express highly in the nucleus. s- lap/ GFP融合蛋白可在人 Hela细胞中表达 ,并主要定位于细胞核。
- The CHO cells that could steadily express at high level the CTLA-4/Ig fusion protein was selected for cloning. 筛选出了能高效稳定表达CTLA-4/Ig融合蛋白的CHO细胞;
- Aftertransected with the expression vector , CHO cells can express the fusion protein stably , which was con-firmed by Western blot and ELISA. Western blot及ELISA法检测到细胞培养上清中有融合蛋白的表达。
- Gpd-Tp92 fusion gene constructed in pcDNA3.1 (+) vector could express a fusion protein in Hela cells. pcDNA3.;1(+)/Gpd-Tp92在HeLa细胞中能有效表达。