The fusion gene was cloned into pcDNA3.1(+) and the recombinant plasmid pcDNA Ag85B MPT64 was transfected into COS 7 cells. The fusion protein expressed in COS 7 cells was determined by RT PCR, ELISA and dot blotting.是什么意思_The fusion gene was cloned into pcDNA3.1(+) and the recombinant plasmid pcDNA Ag85B MPT64 was transfected into COS 7 cells. The fusion protein expressed in COS 7 cells was determined by RT PCR, ELISA and dot blotting.在线翻译_英语_读音_用法_例句