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- Objective To construct the fusion expression vector containing the genes encoding gB680 glycoprotein and mutant pp65(pp65m)protein of human cytomegalovirus(HCMV)and determine the expressed product in COS-7 cells. 目的构建含人巨细胞病毒(HCMV)gB680糖蛋白基因和突变的pp65蛋白基因(pp65m)的融合表达载体,并检测其在COS-7细胞中的表达。
- Keywords RV VP4;Enteotoxigenic Escherichia coli;ST;Fusion expression vector; 轮状病毒VP4;大肠杆菌肠毒素;融合表达载体;
- Construction of fusion expression vector of Ebp 1/EGFP and its expression in HEK293 T cells Ebp1-绿色荧光蛋白融合基因的构建及在HEK293T细胞中的表达
- fusion expression vector 融合表达载体
- Construction of Fusion Expression Vector of Human-derived Neurotrophin-6 Gene Encoding Mature Peptide and Purification of Its Expressed Product 人源性神经营养素-6成熟肽基因融合表达载体的构建及表达产物的纯化
- Objective:To construct and express a recombinant eukaryotic expression vector bearing fusion gene of human IL-2 cDNA gene and Fc fragment. 目的 :构建含人IL 2cDNA基因和免疫球蛋白Fc片段融合基因的真核表达载体pcDNA 3.;1IL 2 /Fc;并在真核细胞中表达;以期用于乙型肝炎病毒 (HBV)DNA疫苗的研究。
- Aftertransected with the expression vector , CHO cells can express the fusion protein stably , which was con-firmed by Western blot and ELISA. Western blot及ELISA法检测到细胞培养上清中有融合蛋白的表达。
- The L ScFv genes were inserted into the eukaryotic fusion protein expression vector pEGFP N3 and transfected into COS 7 cells to express respectively. 将所构建的单链抗体基因克隆入绿色荧光蛋白 (GFP)融合表达载体pEGFP N3,并转染COS 7细胞进行表达。
- The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed. 从活化的人白细胞中克隆到IDO基因编码区全长 ,并构建了IDO EGFP融合蛋白表达载体。
- Inducible prokaryotic expression vector pET-Nus-STK11(with Nus fusion tag) was constructed with pET-44a(+) and the cDNA of STK11 gene cloned in our lab. 利用本室克隆的人STK11 cDNA和原核表达载体pET-44a(+)构建带有Nus融合标签的诱导型表达载体pET-Nus-STK11,在不同的大肠杆菌宿主中诱导表达。
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题: 图1.;穿梭表达载体pRL_hEGF的构建。
- The constructed eukaryotic expression vector contained gB680 and pp65m fusion gene and expressed in COS-7 cells. 重组质粒含有gB680和pp65m融合基因,并能在COS-7细胞中表达。
- The expression vector of PVS CP gene is constructed, a 58kDa fusion protein product is expressed in E. 构建了含PVS CP基因的融合蛋白原核表达载体,并在大肠杆菌(E.;coli
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- Objective To construct the recombinant plasmid of eukaryotic expression vector containing M-CSF gene and transfect it into the NIH3T3 cells to study whether resulting in the expression of M-CSF and EGFP fusion proteins. 目的构建真核表达重组体pEGFP-M-CSF,并鉴定其在小鼠成纤维细胞NIH3T3细胞能否正确表达。
- Restriction endonuclease digestive identification was right for recombinant expression vector pCD11b BFP. Blue fluorescence from fusion protein could be seen in U937 cells transfected with plasmid pCD11b BFP. 经酶切鉴定 ,p CD11b- BFP构建完全正确 ,转染 U937细胞株后 ,可见 CD11b- BFP融合蛋白发出的蓝色荧光。
- Construct rice expression vector of CTB-ureB fusion gene pCWUN:(1) Fusion and clone of CTB gene and ureB gene: CTB gene and Hp ureB gene were amplified by high-fidelity PCR respectively from plasmid pGEM-CTB and Hp genome, and then they were fused by PCR. 构建CTB-ureB融合基因的水稻表达载体pCWUN:
- The fragment containing ubi-53 gene was inserted into pET-28a expression vector, expression of the fusion protein was induced by IPTG in E. Coli BL21 (DE3) . The fusion protein was identified by Western blot with a mouse Ab against bovine Ubiquitin. 将甜菜夜蛾的ubi-53基因克隆到原核表达载体pET-28a上,转化至大肠杆菌BL21(DE3)中,用IPTG进行诱导表达,用异源泛素单克隆抗体进行Western blot检测,证明了原核表达蛋白是目的蛋白。
- Methods Expression vector pCTLA 4/Ig was transfected into COS 7 with lipid reagent. The expression of fusion protein in the supernatant of the cell culture media was detected with double antibody sandwich ELISA. 方法 将pCTLA 4/Ig表达质粒转染COS 7细胞 ,双抗体夹心ELISA检测细胞培养上清中融合蛋白表达 ;