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- The recovered bands of PrP, GFP and pCDNA3.1(-) were ligated with T4 ligase to produce a fusion expression plasmid, pCDNA3.1(-)-mPG, which was then used to transfect the Neuro-2a cell under the selection pressure of G418 antibiotics. 将这三个片段共连接;从而获得融合mPrP/GFP基因的真核表达质粒pCDNA3.;1(-)-mPG。 由于在PrP C-端融合了绿色荧光蛋白(GFP);使该系统具备了早期筛选的标签。
- SUMO protease can cut SUMO fusion protein expressed by fusion expression system without any amino acid residues left on target protein thus become a hot topic in this field. SUMO蛋白酶对SUMO融合表达系统表达的重组蛋白进行切割时没有多余氨基酸残留，因此成为蛋白切割工具的热点。
- Objective To construct the fusion expression vector containing the genes encoding gB680 glycoprotein and mutant pp65(pp65m)protein of human cytomegalovirus(HCMV)and determine the expressed product in COS-7 cells. 目的构建含人巨细胞病毒(HCMV)gB680糖蛋白基因和突变的pp65蛋白基因(pp65m)的融合表达载体,并检测其在COS-7细胞中的表达。
- thioredoxin fusion expression system 硫氧还蛋白融合表达系统
- Keywords rhIL 10;Fusion expression;Inclusion body; 人重组白细胞介素-10;融合表达;包涵体;
- Fusion Expression and Renaturation of Ribonuclease Inhibitor 核糖核酸酶抑制因子的融合表达与复性
- Fusion Expression of Sorbose Dehydrogenase and N-Carbamoylase 山梨糖脱氢酶和氨甲酰化酶的融合表达
- Keywords ribonuclease inhibitor;fusion expression;renaturation; 核糖核酸酶抑制因子;融合表达;复性;
- Americans are the fusion of many different races. 美国人是各种种族人的熔合体。
- Keywords Helicobacter pylori;hpaA gene;cloning;fusion expression; 关键词幽门螺杆菌;粘附素基因;克隆;融合表达;
- Keywords Subclone;Fusion expression;Anti-angiogenesis; 关键词亚克隆;融合表达;抗血管生成;
- An expression of pain flitted across her face. 她脸上闪过一种痛苦的表情。
- Brass is formed by the fusion of copper and zinc. 黄铜是由铜和锌熔合制成的。
- He always shows a doleful expression. 他总是露出忧郁的表情。
- I can think of no greater expression of defeatism. 这是我听到的最悲观的失败主义论调了。
- Keywords Rhodopseudomonas palustris;azoreductase;fusion expression; 沼泽红假单胞菌;偶氮还原酶;融合表达;
- A constant, variable, function name, or expression. 一种常数、变量、函数名或表达式。
- She looked at me without expression. 她毫无表情地朝我看着。
- The scenery there was beautiful beyond expression. 那儿的风景美丽得无法形容。
- He looked at her with a solemn expression. 他表情严肃地望着她。
