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- MethodsThe study was in flask and fermentation tank culture by batch and fed batch method. 方法通过摇瓶培养和分批及补料分批发酵培养进行研究。
- Objective: To prepare recombinant granulysin(GNLY) by fed batch cultivation of E. coli BL21(DE3)pLysS in a FUS-50L bioreactor. 目的:在FUS-50L生物反应器中利用补料分批培养技术培养表达含重组质粒pET28a(+)-GNLY的大肠杆菌BL21(DE3)pLysS株,生产重组颗粒溶素(GNLY)。
- The optimum culture conditions of genetically engineered Pichia pastoris in shake flask cultivation and in fed batch fermentation were investigated respectively. 在摇瓶条件下对基因工程菌 Pichia pastoris的发酵条件进行了实验 ,并根据摇瓶发酵的优化结果进行了补料分批高密度发酵。
- The effect of fed batch system with the measuring tank on the fermentation of a newly selected strain,Aspergillus niger wml-016,was studied in a 50 L automatic fermentor. 选择计量罐式连续流加补料系统,采用新选育的黑曲霉(Aspergillus niger)Wml-016菌株在50 L自动发酵罐上研究间歇补料对发酵的影响。
- The research results shown that: the model obtained could well describe the process of penicillin fermentation in fed batch, as the network architecture is 5 3 5 3 topology, learning step is 1, initial weight is 0.98 and convergence rule is 10 10 . 研究结果表明：当网络拓扑结构为5?3?5?3，步长为1、初始权值取0?98及收敛准则为10?10时，所得到的模型能够很好地描述青霉素补料分批培养过程
- Fed batch fermentation can also increase 1,3 PDO concentration and yield,accompanying the maximal 1,3 PDO concentration and yield,397.7 mmol/L and 0.625 mol/mol is got respectively. 采用流加甘油发酵工艺 ;1 ;3 丙二醇浓度和转化率分别可提高到 397.;7mmol/L和 0
- When the fed batch reaction was made under the above conditions, our results obtained were that concentration of FDP accumulation was 0.399mol/L(135.65g/L) and yield in terms of the glucose consumption was 28.2%. 在该条件下，于补料分批式反应中，FDP积累浓度达到0.;399mol/L（135
- MethodsThe optimized host cell,culture medium and induction time were deternined according to the growth and expression specificity of nrhTNF on test tube and flask shaker. Then fed batch culture was carried out on 5 L automatic fermentor. 方法通过探讨nrhTNF工程菌在试管和锥形摇瓶中的生长和表达规律 ,筛选并确定其最适宿主菌、最佳培养基及最佳诱导表达条件 ,然后在 5L自控发酵罐中进行分批补料培养。
- In fed batch culture,the activity of neutral protease was further increased to 17. 67 g/min L by controlling the feeding rates of glucose and other niedium components. 在控制补加葡萄糖和其它培养基成分的补料分批培养中，中性蛋白酶活性达17.;670g/mm·L。
- Flask-shaking fed batch fermentation is carried out in the study by feeding glucose, ammonium acetate and ammonium sulfate and complete fermentation medium. 进行了补葡萄糖、补醋酸铵和硫酸铵和全组分补料的摇瓶补料分批发酵实验。
- Unwaged members pay a lower entrance fee. 无固定收入的人入场费从优。
- That boxer is a prodigious feed. 那个拳击家是个大肚汉。
- She charged only a nominal fee for her work. 她为所做的工作只收取象征性的费用。
- Study on Fed Batch Fermentation for 2-Keto-L-Gulonate Production 2-酮-L-古洛酸的补料分批发酵的研究
- The fee paid to a mint by a government. 铸币费政府支付给铸币厂的钱
- The ducks of the production team have been fed out. 生产队的鸭子已养肥到可以上市了。
- The second batch of sugar is better than the first. 第二批制成的糖比第一批好。
- Cows and sheep depends forage to feed. 牛和羊依靠草料喂养。
- I thought the accountant's fee rather high. 我认为那个会计师收费很高。
- In batch or remote batch entry, a job or job step. 在批处理或远程批处理输入中的一个作业或作业步。
