Objective: To prepare recombinant granulysin(GNLY) by fed batch cultivation of E. coli BL21(DE3)pLysS in a FUS-50L bioreactor.

• 目的:在FUS-50L生物反应器中利用补料分批培养技术培养表达含重组质粒pET28a(+)-GNLY的大肠杆菌BL21(DE3)pLysS株,生产重组颗粒溶素(GNLY)。