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- Double staining showed two populations of cells with different morphology and color. 双重染色显示两种形态和颜色不同的细胞。
- Objective To identify damaged hair cells in the cochlea by double staining with TUNEL and propidium iodide ( PI ). 目的应用TUNEL与PI(碘化丙啶)双染法检测耳蜗毛细胞损伤。
- Apoptosis was determined by acridine orange(AO) staining,Annexin-V/PI double staining, laser scanning cytometry(LSC) and flow cytometry (FCM). 方法 1.;通过MTT比色法检测NSAIDs对细胞生长活力的影响。 2
- The double staining showed that co expressing rate of CgA/ER and CgA/PR was 10.1%(221/2 208) and 20.1%(490/2 440),respectively. 2 2例CgA阳性子宫内膜癌细胞中CgA /ER同时表达率为 10 .;1%25 (2 2 1/2 2 0 8个细胞 );CgA/PR同时表达率为 2 0
- Gamma-glutamyltranspeptidase (GGT) and alpha fetoprotein(AFP) producing cells in preneoplastic lesions in F_(344) rats were studied by means of double staining of GGT anp AFP. 应用丫-谷氨酰转肽酶-甲胎蛋白(GGT-AFP)双染色法对F_(344)大鼠癌前期病变组织中GGT;AFP的产生细胞进行研究.
- Ponceau 2R-brilliant green double staining technique is a quick,simple and reliable myelin stain method in demonstrating the fasciculi in the central nervous system. 丽春红2R-亮绿法是显示中枢神经纤维束的一种简便、迅速、可靠染色法。
- Conclusions: Ponceau 2R-brilliant green double staining technique is a quick,simple and reliable myelin stain method in demonstrating the fasciculi in the central nervous system. 结论:丽春红2R-亮绿法是显示中枢神经纤维束的一种简便、迅速、可靠染色法。
- Immunocytochemistry was performed by double staining with anti-calcitonin gene-related peptide and anti-NF200 antibodies to evaluate the purification rate of DRG neurons. 采用降钙素基因相关的缩氨酸和神经丝蛋白双重免疫细胞化学染色法鉴定并测定神经元的纯度。
- Immunohistochemical double staining showed that BrdU positive cells could costain with TUNEL, a marker of double strand DNA breaks, and 8-oxoG, an oxidative damage marker. 这提示BrdU+TUNEL双标细胞并不是新生神经元，这反应了缺血早期的BrdU掺入主要标记体内DNA的修复，且Bcl-2在缺血脑组织中的过表达可以影响体内的DNA修复能力。
- A fluorometry method to determine cell viability by double staining with fluorescein diacetste and propidium iodide.Chinese Journal of Cell Biology[J].2000,22(1):50-53. FDA-PI双色荧光分光光度法检测细胞活性变化[J].;细胞生物学杂志;2000;22(1):50-53
- Apoptosis was determined by acridine orange/ethidium bromide(AO/EB) double staining, Annexin V/PI double staining, laser scanning confocul cytometry (LSC), and flow cytometry (FCM). 吖啶橙/溴化乙锭(AO/EB)双染色、AnnexinV/PI双染色、共聚焦显微镜、流式细胞术检测细胞凋亡;
- On the slices with Cx43 and GFAP double labeled,the Cx43 and GFAP double staining positive astrocytes were observed. The GFAP positive astrocytes increased obviously,and the expression of Cx43 also increased obviously in CBX+PTZ group. 在连接蛋白43与胶质纤维酸性蛋白双标的切片上发现,阳性的星形胶质细胞双标,在甘珀酸预处理后的癫痫模型中胶质纤维酸性蛋白阳性的星形胶质细胞显著增加的同时连接蛋白43也明显增加。
- Methods By techniques of cell culture in vitro, annexin-V-FITC and propidium iodide(PI)double staining and flow-cytometry (FCM), RBE cell line was treated by paclitaxel or together with MEK inhibitor PD98059 for 24 h. 方法运用体外细胞培养 ,AnnexinV 异硫氰酸荧光素和碘化丙啶双染法及流式细胞技术观察紫杉醇以及与ERK上游激酶MEK抑制剂PD980 5 9合用 2 4h后RBE细胞的凋亡情况 ,运用蛋白质印迹法观察紫杉醇以及与PD980 5 9合用后RBE细胞的ERK1,ERK2和ERK的活化形式 (pERK)的表达情况。
- Methods HL-60 cells were treated with various concentrations(7.5,15,30, 60mmol/L ) of As4S4.Cell growth was measured by MTT, apoptosis was detected by double staining flow cytometry (FCM). 方法以不同浓度的As4S4(7.;5、15、30、60mmol/L);分别处理24h;48h;72hHL-60细胞;用四甲基偶氮唑蓝(MTT)法测定细胞增殖抑制率; 流式细胞仪(FCM)检测细胞凋亡率;
- Methods Immunohistochemical double staining was used to detect the expression of VEGF-C and podoplanin in 42 paraffin-embedded pancreatic carcinomas and 12 para-carcinomatous tissues. 方法应用免疫组化双标记方法检测42例胰腺癌和12例癌旁胰腺组织中血管内皮生长因子-C(VEGF-C)及肾小球足突细胞黏蛋白(Podoplanin)的表达。
- With the AO/EB double staining, control cells appeared to be round, intact and bright green while some of the exposed cells exhibited irregular cell morphology and condensed nucleus. AO/EB双染结果显示AO/EB双染后细胞的不同形态,其中对照组的细胞呈亮绿色的完整圆球状,而磁场处理后的细胞则呈现出不规则的细胞形态且有凝集的细胞核。
- Double staining involves the use of two stains;the second is called the counterstain. 复合染色过程利用了两种染料，后一种被称为复染剂。
- Double staining with immunohistochemical technology 血管生成双重免疫组织化学染色
- ATPase,NADH-TR and SDH stains demonstrated increase of enzyme activities of parts myofibers. SDH/CCO double stains showed single blue fiber. 酶组织化学 :ATPase、NADH -TR、SDH染色部分肌纤维酶活性增高 ,SDH/CCO双重染色见蓝纤维。
- Immunohistochemical double staining 免疫组化双重染色
