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- ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 采用Dotblot杂交技术定量研究3组胃粘膜ET?1、NOSmRNA表达。
- SCREENING DIFFERENTIALLY EXPRESSED GENES FROM SUPPRESSION SUBTRACTIVE HYBRIDIZATIONAL POSITIVE CLONES USING REVERSE mRNA DOT BLOT TECHNIQUE 利用反向mRNA斑点印迹从抑制消减杂交阳性克隆中筛选差异表达基因
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
- Southern blot technique developed to identify DNA fragments. 印迹杂交被用来发现DNA片段。
- The purpose of this study was evaluation of RT PCR technique,dot blot hybridization with PCR generated probe and SDS PAGE for the detection of rice black streaked dwarf ?Fijivirus?(RBSDV). 用RT PCR技术、PCR标记的探针点杂交和SDS PAGE检测了生产上严重危害玉米和水稻的水稻黑条矮缩病毒(RBSDV)。
- Methods:Oligonucleotide fragment of Brugia malayi synthesized by genetic engineering technique was labeled with 32P and used as probe.The B.malayi larvae in mosquito was detected with dot blot. 方法：利用基因工程技术合成马来丝虫寡核苷酸片段，经32P标记后作为探针，以斑点杂交法检测蚊体内马来丝虫幼虫。
- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶链反应(PCR)结合反向斑点杂交(RDB)技术。
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis进行整合，经G418筛选得到25个高拷贝转化子，经DNA斑点试验和DNA测序证明外源基因E2稳定地整合到P.;Pastoris染色体中。
- Besides, the gene expression of c-myc, wtp53, p16 and EGFR was detected by RNA dot blot. 应用完整细胞原位斑点印迹杂交技术检测c-myc、野生型p53(wtp53)、p16和EGFR的基因表达;
- Denatured-reduced forms of MBP-fusion proteins in immunoblotting, dot blot and ELISA.General. 特异性 Monoclonal Anti-GFP recognizes wild type; recombinant; and enhanced form of GFP.
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的：探讨斑点杂交法检测结核分枝杆菌的临床应用价值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑点杂交证实我们制备的探针是敏感而可靠的。
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑点杂交法和 Southen 印迹法对脑瘤中 erbB、sis、myc 和 fos 这四种癌基因的扩增和重排进行了研究。
- After random labeling with DIG, RNA dot blot was proceed to test the expression of this gene under different growth condition. 用地高辛随机引物标记试剂盒对该片段进行标记后,对不同水分和硫营养条件下提取的小麦根系总RNA 进行斑点杂交。
- Dot blot and southern blot analyses suggested that gfp gene is integrated into the genome of transgenic plants of Nicotiana tabacum, Pentunia genome. 通过对有绿色荧光的烟草、矮牵牛进行dot blot、southern blot分析，都获得阳性杂交结果，证实gfp基因已整合到植物基因组中并与绿色荧光观察结果一致。
- The supernatant of 20% brain tissue suspension of suckling mice infected with BA66019 strain of XHF virus, were separated by electrophoresis of SDS-PAGE and bloted in NC membranes by western blot technique . 用新疆出血热病毒原型毒株BA66019感染乳鼠脑的20%25悬液,经差速离心后的上清为抗原进行SDS-PAGE电泳,再用Westernbolt法将凝胶分离的结果印迹到NC膜上。 将6株单克隆抗体小鼠腹水经饱和硫酸铵纯化后,在NC膜上进行结合反应。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法：采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交（PCR/ASO）技术。
- Methods: In patients with AML M2b, karyotype was studied by using G banding technique, AML1/MTG8 fusion gene transcript by using RT PCR and the rearrangement of AML1 gene and MTG8 gene by using Southern Blot technique. 方法 :应用 G显带技术分析染色体核型 ,用 RT- PCR法检测 AML 1/ MTG8融合基因转录本并进行微量残留白血病检测 ,采用 Southern Blot技术检测 AML 1及 MTG8基因重排。
