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- Keywords embryonic stem cell;progenitor cell;hepatocyte;bile canaliculi like cell;neuron like cell;cytokine;cellular matrix;differentiation; 胚胎干细胞;祖细胞;肝细胞;胆管上皮样细胞;神经样细胞;细胞因子;胞外基质;分化;
- METHODS Paraquat ( PQ ) -induced apoptosis in PC12 cells, which having the characteristics of dopaminergic neuron, as the model of Parkinson disease in vitro. 方法采用百草枯(PQ)诱导的具有多巴胺神经元特性的PC12细胞凋亡作为帕金森病的体外模型。
- cholinergic neuron like cells 胆碱能神经元样细胞
- neuron like cells 神经元样细胞
- After labeled by tyrosine hydroxylase, the differentiated dopaminergic neuron proportion was detected by a flow cytometer. 酪氨酸羟化酶染色标记后通过流式细胞仪检测分化的多巴胺能神经元比率。
- Epidemiological and laboratory data have suggested that A(subscript 2A) blockade may confer neuroprotection against the underlying dopaminergic neuron degeneration. 流行病学研究和实验室研究均表明阻断腺苷A(下标2A)受体能减轻多巴胺能神经元的退行性病变。
- No amplification products was observed in noneuronal wild COS 7 cells, but we can detect the expression of APP gene in COS 7 cell transfected with human APP gene, neuron like wild PC12 cells and human neuroblastoma SH SY5Y cells. 利用专门设计的引物不能检测野生型COS?7细胞中APP基因的表达,但可以检测转染人APP基因的非神经元类COS?7细胞,类神经元的野生型PC12及人神经母细胞瘤细胞SH?SY5Y细胞中APP基因的表达。
- The best expansion conditions for MSC in vitro was 1 l04/ml cell density, 10%FBS, 10ng/ml EGF and 10ng/ml PDGF-BB. After the induction of RA, MSC differentiated into neurons without dopaminergic neuron phenotype. 以接种密度为1×10~4个/ml、加入10%25胎牛血清、10ng/ml EGF和10ng/ml PDGF-BB的培养条件最有利于MSC的增殖;
- Conclusions In vitro, rotenone should be neurotoxic to dopaminergic neuron.It was suggested that rotenone might affect the metabolism of oxidative stress in the pathogenesis of dopaminergic neuron. 结论鱼藤酮在体外对多巴胺能神经元有毒性作用,可抑制细胞增殖,影响线粒体代谢,氧化应激可能在鱼藤酮的多巴胺神经元毒性机制中起作用。
- Objective To investigate the function of amnion endothelial cell in the differentiation of human umbilical cord blood stem cells into dopaminergic neurons. 摘要 目的 探讨羊膜上皮细胞对人脐血干细胞定向分化为多巴胺能神经元的作用。
- Objective:To explore the optimal condition on which embryonic stem cell may be induced to differentiate directly into dopaminergic neurons. 目的:探讨由胚胎干细胞向多巴胺能神经元方向分化的最佳诱导条件。
- OBJECTIVE: This study was designed to observe the differentiation potential of rat mesencephalic neural stem cells at different gestational ages towards dopaminergic neurons. 目的:实验拟观察不同胎龄大鼠中脑神经干细胞体外诱导向多巴胺能神经元分化能力。
- Parkinson's disease arises from genetic and possibly neurotoxic causes that produce massive cell death of the neuromelanin-containing dopaminergic neurons of the substantia nigra. 帕金森氏病的病因包括基因突变和神经毒性物质作用,导致黑质多巴胺能神经元大量的死亡。
- The results of Western blotting were in agreemetn with the immunocytochemistry staining.Conclusion Amnion endothelial cells can induce human umbilical cord blood stem cells into dopaminergic neurons. 结论 羊膜上皮细胞能诱导人脐血间充质干细胞定向分化为多巴胺能神经元。
- Objective To study the apoptosis effects and the mechanisms of action by homocysteine on dopaminergic neurons. 目的:研究同型半胱氨酸能否通过内质网应激的途径诱导多巴胺能神经元凋亡。
- ASCs could be differentiated into myocardial like cells induced by myocardial cell lysate. 心肌细胞裂解液可在体外诱导ASCs分化为类心肌细胞。
- Abstract: Objective To study the toxicity of exogenous dopamine(DA) in dopaminergic neurons. 摘 要: 目的研究外源性多巴胺对多巴胺能神经元的毒性作用。
- Mononuclear stroma cells were either fibroblast like cells or histocyte like cells. 单核基质细胞可分为两种类型,以纤维母细胞型占优势,主要表达波形蛋白和PCNA;
- In many parkinson's cases, the damage can be seen in autopsies as clumps of proteins within the substantia nigra's dopaminergic neurons. 解剖许多帕金森氏症患者的尸体后发现,黑质多巴胺神经元中有堆积成块的蛋白质。
- The medial forebrain bundle of rats was transected with a wire knife to set up a model of chronic degeneration of dopaminergic neurons. 采用线刀切断大鼠内侧前脑束造成多巴胺能神经元的慢性退行性改变。