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- Results In PCR, the primer for internal reference gene, content of dNTP, and runing cycles affect the products much than the annealing temperature. 结果在pcr反应中,扩增内参照和目的基因的引物比例、底物浓度以及pcr反应的循环次数对产物的影响较大,而退火温度的影响较小。
- We optimized the concentration of Mg2+ ,dNTP and primers in PCR reaction mixture by amplifying the RNAtranscribed in vitro above. 对 RT-PCR 反应体系中的 Mg2+,dNTP 和引物浓度进行优化。
- This suggests that harringtonine might block the polymerization of dNTP into DNA, but without influence on the phosphorylation of TdR. 说明三尖杉酯碱不抑制胸腺嘧啶核苷的磷酸化,而抑制脱氧核苷三磷酸到DNA的聚合过程。
- Method of DNA extraction and factors influencing RAPD analysis,including the concentration of DNA template, Mg 2+ , dNTP, primers and Taq, in jute were studied. 以假黄麻、假长果、越南圆果 (圆果种黄麻 )等为材料 ;研究了黄麻 DNA的提取方法以及对 RAPD分析的影响因素 ;包括模板浓度、Mg2 + 、d NTP、引物和 Taq酶等 ;建立了适于黄麻种质 RAPD分析的 PCR反应体系 .
- Factors which affect the ISSR analysis of Abies beshanzuensis,such as annealing temperature and concentrations of template DNA,Taq DNA polymerase,Mg~2+ and dNTP,etc. were examined. 以百山祖冷杉DNA为材料;分析了DNA浓度、Mg2+浓度、dNTP浓度、Taq酶的含量以及退火温度对ISSRPCR扩增结果的影响;经过优化实验建立了百山祖冷杉ISSRPCR最佳反应体系.
- The ladder experiments showed: the best PCR amplified system of the crested ibis is as follows: Primer(10pmol/ul)lul, Taq enzyme(lU/ul)1.5ul, dNTP(2.5mM)2.0ul, Mg2+(25mM)2.5ul, teplate DNAlOng, TH2O17ul, and final volume is 25ul. 本试验对各种影响因素进行了梯度筛选，得到朱?最佳的PCR扩增体系为：引物(10pmol/ul)1ul、TaqDNA聚合酶(1U/ul)1.;5ul、dNTP(2
- deoxyribonucleoside triphosphate(dNTP) 脱氧核糖核苷三磷酸
- dNTP concentration grads dNTP浓度梯度
- dinitrothiophosphate 二硝基硫代磷酸盐(酯)
- dNTP (=deoxy-ribonucleoside triphosphate) 三磷酸脱氧核(糖核)苷
- DNTP(parathion) 对硫磷[杀虫药]
- cDNA was synthesized by reverse transcription (RT). The total volume of reaction system was 20ulincluded 4uL MgCL2, 2ul IQxRNAPCRbuffer, 2ul dNTP mixture, O. Sul Rnase Inhibitor, lul Random 9mers, lul Reverse Transcriptase, lug sample. 逆转录(RT)合成cDNA，反应体系20UL包括：MgCL_24uL，10×RNA PCR缓冲液2ul，dNTP混合液2ul，RNA酶抑制物0.;5ul，随机引物1ul，逆转录酶1ul，样品RNA 1ug。
