cDNA was synthesized by reverse transcription (RT). The total volume of reaction system was 20ulincluded 4uL MgCL2, 2ul IQxRNAPCRbuffer, 2ul dNTP mixture, O. Sul Rnase Inhibitor, lul Random 9mers, lul Reverse Transcriptase, lug sample.

• 逆转录(RT)合成cDNA，反应体系20UL包括：MgCL_24uL，10×RNA PCR缓冲液2ul，dNTP混合液2ul，RNA酶抑制物0.；5ul，随机引物1ul，逆转录酶1ul，样品RNA 1ug。