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- The amplicons can be separated by polyacrylamide gel, denaturing polyacrylamide gel or agarose gel, and detected by autoradiography, silver or EB staining. 扩增产物可在聚丙烯酰胺凝胶、变性聚丙烯酰胺凝胶、琼脂糖凝胶上进行电泳分离,检测时可使用同位素、银染或溴化乙锭染色等技术。
- The amplification fragment length polymorphisms (Amp-FLPs) of short tandern repeat (STR) locus ACTBP2 were studied by polymerase chain reaction (PCR) technique and denatured polyacrylamide gel electrophoresis(PAGE) followed by silver staining. 应用变性聚丙烯酸胺凝胶电泳(dn-PAGE)结合银染色技术对短串联重复(STR)位点ACTBP2(SE33)的扩增片段长度多态性(Amp-FLPs)进行了研究。
- Methods Afragment f 287 bp Alu sequence in inron 16 of ACE gene was used as I/D polymorphic marker. After PCR amplification of DNA fragment,12% non denatured polyacrylamide gel electrophoresis was undertaken to analyse the PCR products. 方法以ACE基因内含子16的一个287bp的Alu顺序I/D型为多态标志,用聚合酶链反应(PCR)扩增基因片段,12%25非变性聚丙烯酰胺凝胶电泳检测PCR产物。
- Methods The mRNA expression of five MMR genes and six microsatellite markers were detected in 46 primary lung cancer tissues by RT PCR and PCR denature polyacrylamide gel electrophoresis silver staining methods. 方法 用RT PCR和PCR -变性聚丙烯酰胺凝胶电泳 -银染法 ,检测 46例原发性肺癌组织中 5种MMR基因mRNA表达及 6种微卫星改变。
- Methods The two microsatellite markers were amplified by polymerase chain reaction(PCR), followed by 10% non denatured polyacrylamide gel electrophresis (PAGE) and DNA sequencing to analyze the PCR products. 方法用聚合酶链反应(PCR)扩增与PKD1连锁的两个微卫星,10%25非变性聚丙烯酰胺凝胶电泳结合DNA序列测定检测PCR产物。
- non - denaturant polyacrylamide gel eletrophoresis 非变性聚丙烯酰胺凝胶
- denaturing polyacrylamide gel 变性聚丙烯酰胺凝胶
- As analyzed on polyacrylamide gel electrophoresis in denaturing conditions (SDS-PAGE), CRL A and CRL B both have the same molecular weight range of 62000-64000Da. SDS聚丙烯酰胺凝胶电泳分析发现,CRL A和CRL B的分子量几乎相同(约为62000~64000Da)。
- Clinical application of non- denaturant polyacrylamide gel electrophoresis and silver staining test 非变性聚丙烯酰氨凝胶电泳及银染法临床应用探讨
- Methods: Using polyacrylamide gel electrophoresis ( PAGE). 方法:垂直板电泳法。
- denaturing gradient polyacrylamide gel 变性梯度聚丙烯酰胺凝胶
- This paper dealt with polyacrylamide gel electrophoresis( PAGE) of lactate dehydrogenase( LDH) isoenzymes in fish. 本文对用聚丙烯酰胺凝胶电泳(AGE)离鱼类乳酸脱氢酶(DH)工酶的技术进行了研究。
- Extracts of the hybrids were then subjected to polyacrylamide gel electrophoresis and tested for TK activity. 用聚丙烯酰胺凝胶电泳检测杂种提取种物的TK活力。
- The gliadin patterns of 9 Triticum polonicum and 2 T. petropavlovskyi were analyzed by acid polyacrylamide gel electrophores is (APAGE) method. 利用酸性聚丙烯酰胺凝胶电泳(APAGE)对9份波兰小麦和2份新疆稻麦进行了醇溶蛋白分析。
- Methods: Pot control was adopted in the experiments and vertical slab polyacrylamide gel electrophoresis technique was utilized in the POD analysis. 方法采用盆栽控制试验,利用垂直板聚丙烯酰胺凝胶电泳分析技术进行同工酶分析。
- Its molecular weight is 132,000 dalton by sodium dodecylsulfate (SDS) polyacrylamide gel method. 按SDS聚丙烯酰胺凝胶电泳(SDS-PAGE)测得其分子量为132,000道尔顿。
- S-protein was isolated from RNase A by polyacrylamide gel electrophoresis afterproteolytic digestion with subtilisin. 核糖核酸酶A具有DNA结合蛋白的作用。
- Objective:To analyze the hematoma complicated by injectable polyacrylamide gel au gmentation mammoplasty. 目的:探讨聚丙烯酰胺水凝胶注射隆乳术并发血肿的发生与预防措施。
- Firstly, nuclease P1 was embeded in polyacrylamide gel, and couple-linked to chitosan with glutaraldehyde. 首先,分别以聚丙烯酰胺包埋、壳聚糖偶联固定核酸酶P1。
- The peptides were extracted, and analyzed using an improved tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis (Tricine-SDS-PAGE) method. 方法通过针刺感染大肠杆菌诱导家蝇各虫态产生抗菌肽,再经研磨、离心等步骤提取这些抗菌肽,用改进的聚丙烯酰胺凝胶电泳使其分离。