Methods Afragment f 287 bp Alu sequence in inron 16 of ACE gene was used as I/D polymorphic marker. After PCR amplification of DNA fragment,12% non denatured polyacrylamide gel electrophoresis was undertaken to analyse the PCR products.

• 方法以ACE基因内含子16的一个287bp的Alu顺序I/D型为多态标志，用聚合酶链反应（PCR）扩增基因片段，12%25非变性聚丙烯酰胺凝胶电泳检测PCR产物。