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- Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基于生物信息学分析构建人ID4基因启动子表达载体,以其作为研究ID4基因启动子表达调控分析的工作基础。
- Optimized-code bull protamine gene was synthesized, the code (CGT) which E. coli. is partial to was substituted for the rare codes(AGA or AGG), the gene was cloned into pGEX-2T to construct expression vector pGEX-2T-PS. 利用大肠杆菌偏好的编码精氨酸的密码子CGT将野生型牛精蛋白基因中编码精氨酸的稀有密码子 (AGA或AGG)替换掉 ,通过基因合成得到密码子优化的牛精蛋白基因 ,将其克隆入原核表达载体pGEX 2T ,组装成表达载体pGEX 2T PS。
- The main methods and results are as follows: 1.The constructed expression vector, pMSCV-ncam, was constructed and proved correct by enzyme restriction pattern. 2. 实验方法结果如下: 1.;对已构建含有NCAM的质粒载体进行酶切鉴定,证明表达载体构建正确,转化入细菌(TOP10),扩增,纯化,备用。
- A new upsurge in construction is in the making. 一个建设的新高潮正在兴起。
- Can you cost out this construction work? 你能估计这项工程的成本吗?
- The house/highway is in course of construction. 该房子/公路正在施工中。
- Construction is a major industry here. 建筑业是此地的主要工业。
- There are two new houses under construction. 有两座新房子正在修建。
- A dump truck was parked on the construction site. 建筑工地上停著一辆倾卸车。
- The building is a construction of wood. 这是木质结构的建筑。
- Let's put a good construction on her conduct first. 让我们对她的行为先作一个善意的解释吧。
- A new railroad is under construction. 一条新的铁路正在修建中。
- This highway is in course of construction now. 这条公路目前正在修建中。
- The new school is still under construction. 新学校还在建造中。
- Pure and complex characters of constructing expression 建筑表达的纯粹性与复杂性
- How did the construction work bog down? 建筑工程怎样会停下来的?
- Heavy snow hindered the construction work. 大雪妨碍了这项建筑工程的进行。
- Somehow the engineers screwed up the entire construction project. 不知怎么搞的,工程师把整个工程计划弄得一塌糊涂。
- A construction or form in the active voice. 主动语态结构,主动语态形式主动语态的结构或形式
- The next step is to plan construction of the highway. 下一步就是制定公路的建设计划。